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 Title: |
US5514537:
Process and apparatus for sorting spermatozoa
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Chandler, John E.; Baton Rouge, LA
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| Assignee: |
Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, Baton Rouge, LA
other patents from LOUISIANA STATE UNIVERSITY (343025) (approx. 62)
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| Published / Filed: |
1996-05-07
/ 1994-11-28
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| Application Number: |
US1994000347793
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| IPC Code: |
Advanced:
A61K 35/52;
Core:
A61K 35/48;
IPC-7:
A01N 1/02;
A61K 35/52;
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| ECLA Code: |
A61K35/52;
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| U.S. Class: |
Current:
435/002;
424/561;
436/906;
Original:
435/002;
424/561;
436/906;
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| Field of Search: |
435/002
210/656,702
424/561
436/063,807,906
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| Priority Number: |
| 1994-11-28 |
US1994000347793 |
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| Abstract: |
A method and apparatus are disclosed for the mechanical sorting of mammalian spermatozoa by sex-type, into a fraction enriched in X-chromosome-bearing spermatozoa, and a fraction enriched in Y-chromosome-bearing spermatozoa. Because of their different DNA content, Y-chromosome spermatozoa are slightly smaller than X-chromosome spermatozoa. A column is packed with two sizes of beads. The size of the smaller beads is chosen such that, on average, Y-chromosome spermatozoa will readily fit into the interstices between the smaller beads, while X-chromosome spermatozoa, on average, will not readily fit into those interstices. The size of the larger beads is chosen such that the smaller beads will not readily fit into the interstices between the larger beads. A liquid sample containing the sperm is run through a column so that the liquid first encounters the larger beads, and then encounters the smaller beads. The beads act as a sieve, creating a fraction in the larger beads enriched in X-chromosome spermatozoa, and a fraction in the smaller beads enriched in Y-chromosome spermatozoa.
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| Attorney, Agent or Firm: |
Runnels, John H. ;
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| Primary / Asst. Examiners: |
Gitomer, Ralph J.;
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| Maintenance Status: |
E3 Expired Check current status
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| INPADOC Legal Status: |
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| Family: |
None
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First Claim:
Show all 15 claims |
I claim:
1. An apparatus for sorting sperm from a mammalian species into a first fraction enriched in sperm bearing an X chromosome and a second fraction enriched in sperm bearing a Y chromosome, said apparatus comprising a column, a plurality of first beads, and a plurality of second beads, wherein:
- (a) said first beads are tightly packed in a first region of said column;
- (b) said second beads are tightly packed in a second region of said column;
- (c) said first and second regions are distinct regions within said column, said first and second regions being adjacent to one another;
- (d) said second beads are substantially spherical, and are substantially uniform in radius, wherein the second beads have a radius such that a length equal to 0.155 times the second bead radius is intermediate in magnitude between the mean radius of the mammalian species' sperm containing an X chromosome, and the mean radius of the mammalian species' sperm containing a Y chromosome; and
- (e) said first beads are substantially spherical, and are substantially uniform in radius, wherein the first beads have a radius greater than the second bead radius.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 15 U.S. patent(s) that reference this one
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| Foreign References: |
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| Other Abstract Info: |
DERABS C96-238761
DERC96-238761
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| Other References: |
Daya S., Separation of Motile Human Spermatozoa . . . Gamete Research 17: 375-380 (1987).
(6 pages)
Casey P., Column Separation of Motile Sperm . . . Andrology (14) 2 Mar./Apr. (1993) pp. 142-148.
(7 pages)
[ISI abstract]
K. Cui et al., "X Larger than Y," Nature, vol. 366, pp. 117-118 (Nov. 1993).
(2 pages)
[ISI abstract]
Chandler et al., "Videomicroscopic Comparison of Bull Sperm and Leukocyte Chromosome Areas as related to Gender," J. Dairy Sci., vol. 73, pp. 2129-2135 (1990).
(7 pages)
Cited by 6 patents
B. L. Gledhill et al., "Identifying X- and Y-Chromosome-Bearing Sperm by DNA Content: Retrospective Perspectives and Prospective Opinions, " in R. P. Amann et al. (eds.) Prospects for Sexing Mammalian Sperm, pp. 177-191 (1982).
W. A. Wells et al., "Equipment, Standardization and Applications of Image Processing," Amer. J. Clin. Path., vol. 99, pp. 48-56 (1993).
(9 pages)
[ISI abstract]
D. G. Cran et al., "Production of Bovine Claves Following Separation of X- and Y-Chromosome Bearing Sperm and In Vitro Fertilisation," Veterinary Record, vol. 132, pp. 40-41 (1993).
(2 pages)
Cited by 8 patents
[ISI abstract]
Fike et al., "Preparative Cell Electrophoresis," Preparative Biochemistry, vol. 3, No. 2, pp. 183-193 (1973).
(11 pages)
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