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 Title: |
US6194191:
Method for the production and purification of adenoviral vectors
[ Derwent Title ]
>> View Certificate of Correction for this publication
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| Country: |
US United States of America
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| Inventor: |
Zhang, Shuyuan; Sugar Land, TX
Thwin, Capucine; Spring, TX
Wu, Zheng; Sugar Land, TX
Cho, Toohyon; Houston, TX
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| Assignee: |
Introgen Therapeutics, Inc., Austin, TX
other patents from INTROGEN THERAPEUTICS, INC. (779615) (approx. 5)
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| Published / Filed: |
2001-02-27
/ 1997-11-20
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| Application Number: |
US1997000975519
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| IPC Code: |
Advanced:
A61K 35/76;
C07K 14/47;
C12N 7/01;
C12N 7/02;
C12N 15/861;
A61K 48/00;
Core:
A61K 35/66;
C07K 14/435;
more...
IPC-7:
C12N 7/01;
C12N 7/02;
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| ECLA Code: |
A61K35/76; C07K14/47A32; C12N7/02; C12N15/861; K61K48/00; M07K221/04; M12N710/19A;
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| U.S. Class: |
Current:
435/239;
424/199.1;
435/235.1;
435/320.1;
Original:
435/239;
435/235.1;
435/320.1;
424/199.1;
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| Field of Search: |
435/235.1,239,320.1,366,369,383
424/199.1
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| Priority Number: |
| 1997-11-20 |
US1997000975519 |
| 1996-11-20 |
US1996000031329P |
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| Abstract: |
The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
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| Attorney, Agent or Firm: |
Fulbright & Jaworski ;
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| Primary / Asst. Examiners: |
Mosher, Mary E.;
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| Maintenance Status: |
CC Certificate of Correction issued
View Certificate of Correction
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| INPADOC Legal Status: |
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Family Legal Status Report
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| Parent Case: |
The present application is a continuation-in-part of co-pending U.S. Provisional Patent Application Ser. No. 60/031,329 filed Nov. 20, 1996. The entire text of the above-referenced disclosure is specifically incorporated by reference herein without disclaimer.
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| Designated Country: |
AL AM AP AZ BA BB BG BY CA CU CZ EE GE GH HU ID IL IS KE KG KP KR KZ AT BE CH DE DK ES FI FR GB GR IE IT
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| Family: |
Show 32 known family members
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First Claim:
Show all 89 claims |
What is claimed is:
1. A method for producing a purified adenovirus composition comprising:
- a) growing host cells in a media;
- b) providing nutrients to said host cells by perfusion or through a fed-batch process;
- c) infecting said host cells with an adenovirus;
- d) lysing said host cells to provide a cell lysate comprising adenovirus, wherein said lysis is achieved through autolysis of infected cells; and
- e) purifying adenovirus from said lysate to provide a purified adenovirus composition.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
Show description
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| Forward References: |
Show 26 U.S. patent(s) that reference this one
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| Foreign References: |
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| Other Abstract Info: |
CHEMABS 129(03)024181T
DERABS C1998-312474
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| Other References: |
T. Cartwright. Animal Cells as Bioreactors. Cambridge University Press. pp. 58-63, "Fermenter design for animal cell cultures", 1994.
Provisional U.S. Patent Application Ser. No. 60/026,667, Entitled: "Method for the Production of Recombinant Adenoviruses," RPR File No. ST96021-U.S., Translated from the French by the Medical Documentation Service® Institute for Scientific Information® Philadelphia, Pennsylvania.
Aboud et al., "Rapid purification of extracellular and intracellular moloney murine leukemia virus," Arch. Virol., 71:185-195, 1982.
(11 pages)
Cited by 4 patents
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(7 pages)
Cited by 15 patents
[ISI abstract]
Bett, "An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3," Proc. Natl. Acad. Sci. USA, 91(19):8802-8806, 1994.
(5 pages)
Cited by 68 patents
[ISI abstract]
Crooks et al., "Purification and analysis of infections virions and native non-structural antigens from cells infected with tick-borne encephalitis virus," J. Chrom., 502:59-68, 1990.
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(11 pages)
Cited by 12 patents
[ISI abstract]
Gilbert, "Adaptation of cells to serum free culture for production of adenovirus vectors and recombinant proteins," Williamsburg BioProcessing Conference, Nov. 18-21, 1996.
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Graham et al, "Characteristics of a human cell line transformed by DNA from human adenovirus type 5," J. Gen. Virol., 36:59-72, 1977.
(14 pages)
Cited by 200 patents
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Hay et al., "Replication of adenovirus mini-chromosomes," J. Mol. Biol., 175:493-510, 1984.
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(14 pages)
Cited by 5 patents
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(4 pages)
Cited by 31 patents
Huyghe et al., "Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography," Hum. Gene Ther., 6:1403-1416, 1996.
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(8 pages)
Cited by 15 patents
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Cited by 131 patents
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Cited by 10 patents
[ISI abstract]
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[ISI abstract]
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[ISI abstract]
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(11 pages)
Cited by 5 patents
[ISI abstract]
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| Continuity Data: |
| Application Number | Filed | Notes |
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US1997000975519 | 1997-11-20 | is a
related to the prior publication |
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US20020177215A1 issued 2002-11-28 Methods for producing purified adenoviral vectors
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US1997000975519 | 1997-11-20 | is a
related to the prior publication |
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US20020182723A1 issued 2002-12-05 AN IMPROVED METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS
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US1997000975519 | 1997-11-20 | is a
related to the prior publication |
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US20050089999A1 issued 2005-04-28 Method for the production and purification of adenoviral vectors
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US2006000508780 | 2006-08-23 | is a
continuation of |
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US2004000909066
| 2004-07-30 |
(pending)
[presumed granted]
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US7445930 issued 2008-11-04 Method for the production and purification of adenoviral vectors
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US2004000909066 | 2004-07-30 | is a
division of |
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US2001000033571
| 2001-12-27 |
(pending)
[presumed granted]
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US7510875 issued 2009-03-31 Methods for producing purified adenoviral vectors
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US2004000909066 | | is a
division of |
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US2001000033571
| 2001-12-27 |
(pending)
[presumed granted]
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US7510875 issued 2009-03-31 Methods for producing purified adenoviral vectors
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US2002000033571 | | is a
continuation of |
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US2000000556570
| 2000-04-24 |
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US6726907 issued 2004-04-27 Purified adenoviral compositions
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US2001000033571 | 2001-12-27 | is a
continuation of |
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US2000000556570
| 2000-04-24 |
(pending)
[presumed granted]
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US6726907 issued 2004-04-27 Purified adenoviral compositions
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US2001000033571 | 2001-12-27 | is a
continuation of |
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US2000000556570
| 2000-04-24 |
(granted)
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US6726907 issued 2004-04-27 Purified adenoviral compositions
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US2001000033491 | 2001-12-27 | is a
continuation of |
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US2000000556570
| 2000-04-24 |
(pending)
[presumed granted]
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US6726907 issued 2004-04-27 Purified adenoviral compositions
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US2001000880609 | 2001-06-12 | is a
division of |
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US1998000203078
| 1998-12-01 |
(pending)
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US2001000880609 | | is a
division of |
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US1998000203078
| 1998-12-01 |
(pending)
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US2000000556570 | 2000-04-24 | is a
continuation of |
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>US1997000975519<
| 1997-11-20 |
(granted)
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US6194191 issued 2001-02-27 An improved method for the production and purification of adenoviral vectors
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|
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US2000000556570 | | is a
continuation of |
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>US1997000975519<
| 1997-11-20 |
|
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US6194191 issued 2001-02-27 An improved method for the production and purification of adenoviral vectors
|
|
|
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US1998000203078 | | is a
continuation in part of |
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>US1997000975519<
| 1997-11-29 |
(pending)
[presumed granted]
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US6194191 issued 2001-02-27 An improved method for the production and purification of adenoviral vectors
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US1998000203078 | 1998-12-01 | is a
continuation in part of |
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>US1997000975519<
| 1997-11-20 |
(granted)
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US6194191 issued 2001-02-27 An improved method for the production and purification of adenoviral vectors
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US1997000975519 | 1997-11-20 | is a
non-provisional of provisional |
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US1996000031329P
| 1996-11-20 |
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