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 Title: |
US3853832:
SYNTHETIC HUMAN PITUITARY GROWTH HORMONE AND METHOD OF PRODUCING IT
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| Country: |
US United States of America
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| Inventor: |
Li, Choh H.; Berkeley, CA
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| Assignee: |
Harmone Research Foundation, Berkeley, CA
other patents from HORMONE RESEARCH FOUNDATION (257415) (approx. 2)
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| Published / Filed: |
1974-12-10
/ 1971-04-27
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| Application Number: |
US1971000137811
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| IPC Code: |
Advanced:
C07K 1/00;
C07K 7/06;
IPC-7:
C07C 103/52;
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| ECLA Code: |
C07K7/06; C07K1/00B;
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| U.S. Class: |
Current:
530/399;
530/334;
530/404;
930/120;
930/260;
Original:
260/112.5;
424/177;
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| Field of Search: |
260/112.5
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| Priority Number: |
| 1971-04-27 |
US1971000137811 |
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| Abstract: |
Synthetic human pituitary growth hormone (HGH) is produced by attaching phenylalanine, the COOH terminal amino acid of HGH, to a solid resin support, and then coupling stepwise to the supported acid the remaining 187 amino acid residues of the HGH molecule, using the general technique of solid phase peptide synthesis with suitable protective and blocking groups, and deblocking and coupling agents. The resulting synthetic polypeptide chain of 188 amino acid residues is liberated from the resin support and all protecting groups are removed. Then the synthetic hormone is oxidized under controlled conditions effective to form two disulfide bridges across the cysteine residues in the chain, thereby forming the two intra-molecular rings which are required to reproduce the correct conformation of the HGH molecule. The resulting synthetic hormone reacts immunologically with rabbit antiserum to HGH and possesses growth-promoting and lactogenic activity.
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| Attorney, Agent or Firm: |
Pennie & Edmonds ;
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| Primary / Asst. Examiners: |
Roberts, Elbert L.;
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| INPADOC Legal Status: |
None
Family Legal Status Report
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| Family: |
Show 10 known family members
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First Claim:
Show all 20 claims |
I claim:
1. A method of producing a synthetic human growth - promoting substance which comprises:
- a. forming an unbridged polypeptide chain of 188 amino acid residues in the sequence shown in the accompanying drawing;
- b. generating sulfhydryl groups on the cysteine residues in said polypeptide chain;
- c. oxidizing said sulfhydryl groups under conditions effective to form disulfide bridges between said cysteine residues, thereby forming two intra-molecular rings in the polypeptide chain.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 17 U.S. patent(s) that reference this one
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