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 Title: |
US4237224:
Process for producing biologically functional molecular chimeras
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Cohen, Stanley N.; Portola Valley, CA
Boyer, Herbert W.; Mill Valley, CA
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| Assignee: |
Board of Trustees of the Leland Stanford Jr. University, Stanford, CA
other patents from STANFORD UNIVERSITY (675809) (approx. 1,667)
News, Profiles, Stocks and More about this company
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| Published / Filed: |
1980-12-02
/ 1979-01-04
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| Application Number: |
US1979000001021
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| IPC Code: |
Advanced:
C12N 15/00;
Core:
more...
IPC-7:
C12P 21/00;
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| ECLA Code: |
C12N15/00;
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| U.S. Class: |
Current:
435/069.1;
435/069.2;
435/069.3;
435/069.4;
435/069.5;
435/069.51;
435/069.52;
435/069.6;
435/091.1;
435/091.4;
435/091.41;
435/183;
435/207;
435/212;
435/231;
435/252.33;
435/320.1;
435/820;
435/849;
530/311;
530/397;
530/399;
530/808;
536/023.1;
Original:
435/068;
435/172;
435/231;
435/183;
435/317;
435/849;
435/820;
435/091;
435/207;
260/112.5S;
260/027;
435/212;
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| Field of Search: |
195/001,28 N,28 R,112,78,79
435/068,172,231,183
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| Government Interest: |
The invention was supported by generous grants of NIH, NSF and the American Cancer Society.
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| Priority Number: |
| 1979-01-04 |
US1979000001021 |
| 1978-11-09 |
US1978000959288 |
| 1976-05-17 |
US1976000687430 |
| 1974-11-04 |
US1974000520691 |
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| Abstract: |
Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini to which is inserted a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids and proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like.
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| Attorney, Agent or Firm: |
Rowland, Bertram I. ;
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| Primary / Asst. Examiners: |
Tanenholtz, Alvin E.;
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| INPADOC Legal Status: |
None
Family Legal Status Report
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| Related Applications: |
| Application Number |
Filed |
Patent |
Pub. Date |
Title |
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| US1978000959288 | 1978-11-09 |
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| US1976000687430 | 1976-05-17 |
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| US1974000520691 | 1974-11-04 |
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| Parent Case: |
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuatin-in-part of applicatin Ser. No. 959,288, filed Nov. 9, 1978, which is a continuation of application Ser. No. 687,430 filed May 17, 1976, now abandoned, which was a continuation-in-part of application Ser. No. 520,691, filed Nov. 4, 1974, now abandoned.
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| Family: |
Show 3 known family members
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First Claim:
Show all 14 claims |
We claim:
1. A method for replicating a biologically functional DNA, which comprises:
- transforming under transforming conditions compatible unicellular organisms with biologically functional DNA to form transformants; said biologically functional DNA prepared in vitro by the method of:
- (a) cleaving a viral or circular plasmid DNA compatible with said unicellular organism to provide a first linear segment having an intact replicon and termini of a predetermined character;
- (b) combining said first linear segment with a second linear DNA segment, having at least one intact gene and foreign to said unicellular organism and having termini ligatable to said termini of said first linear segment, wherein at least one of said first and second linear DNA segments has a gene for a phenotypical trait, under joining conditions where the termini of said first and second segments join to provide a functional DNA capable of replication and transcription in said unicellular organism;
- growing said unicellular organisms under appropriate nutrient conditions; and
- isolating said transformants from parent unicellular organisms by means of said phenotypical trait imparted by said biologically functional DNA.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
Show drawing descriptions
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| Description: |
Show description
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| Forward References: |
Show 294 U.S. patent(s) that reference this one
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 094(09)061591F
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| Other References: |
Morrow et al., Proc. Nat. Acad. Sci. USA, vol. 69, pp. 3365-3369, Nov. 1972.
Morrow et al., Proc. Nat. Acad. Sci. USA, vol. 71, pp. 1743-1747, May 1974.
(5 pages)
Cited by 6 patents
Hershfield et al., Proc. Nat. Acad. Sci. USA, vol. 71, pp. 3455 et seq. (1974).
(5 pages)
Cited by 8 patents
Jackson et al., Proc. Nat. Acad. Sci. USA, vol. 69, pp. 2904-2909, Oct. 1972.
Cited by 7 patents
Mertz et al., Proc. Nat. Acad. Sci. USA, vol. 69, pp. 3370-3374, Nov. 1972.
Cited by 4 patents
Cohen, et al., Proc. Nat. Acad. Sci. USA, vol. 70, pp. 1293-1297, May 1973.
(5 pages)
Cohen et al., Proc. Nat. Acad. Sci. USA, vol. 70, pp. 3240-3244, Nov. 1973.
(5 pages)
Cited by 15 patents
Chang et al., Proc. Nat. Acad. Sci, USA, vol. 71, pp. 1030-1034, Apr. 1974.
(5 pages)
Cited by 2 patents
Ullrich et al., Science vol. 196, pp. 1313-1319, Jun. 1977.
Singer et al., Science vol. 181, p. 1114 (1973).
(1 pages)
Cited by 4 patents
Itakura et al., Science vol. 198, pp. 1056-1063 Dec. 1977.
(8 pages)
Cited by 180 patents
Komaroff et al., Proc. Nat. Acad. Sci. USA, vol. 75, pp. 3727-3731, Aug. 1978.
Chemical and Engineering News, p. 4, May 30, 1977.
Chemical and Engineering News, p. 6, Sep. 11, 1978.
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