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 Title: |
US4332892:
Protein synthesis
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Ptashne, Mark; Cambridge, MA
Lauer, Gail D.; Cambridge, MA
Roberts, Thomas M.; Cambridge, MA
Backman, Keith C.; San Francisco, CA
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| Assignee: |
President and Fellows of Harvard College, Cambridge, MA
other patents from HARVARD COLLEGE, PRESIDENT AND FELLOWS (242920) (approx. 503)
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| Published / Filed: |
1982-06-01
/ 1980-01-10
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| Application Number: |
US1980000111101
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| IPC Code: |
Advanced:
C12N 15/00;
C12N 15/72;
Core:
more...
IPC-7:
C12P 21/00;
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| U.S. Class: |
Current:
435/069.7;
435/488;
Original:
435/068;
435/070;
435/172;
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| Field of Search: |
435/172,70,71,68
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| Priority Number: |
| 1980-01-10 |
US1980000111101 |
| 1979-01-15 |
US1979000003102 |
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| Abstract: |
This invention is a process to produce specific proteins coded for by eukaryotic (or prokaryotic) DNA in bacteria. The invention, which uses recombinant DNA techniques, produces proteins in their natural, functional state unencumbered by extraneous peptides.
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| Primary / Asst. Examiners: |
Tanenholtz, Alvin E.;
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| INPADOC Legal Status: |
None
Family Legal Status Report
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| Parent Case: |
This is a continuation of application Ser. No. 3,102, filed Jan. 15, 1979, and now abandoned.
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| Family: |
Show 9 known family members
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First Claim:
Show all 6 claims |
What is claimed is:
1. The method of producing native, unfused prokaryotic or eukaryotic protein in bacteria which comprises inserting into a bacterial plasmid a gene for a prokaryotic or eukaryotic protein and a portable promoter consisting of a DNA fragment containing a Shine-Dalgarno sequence and a transcription initiation site recognized by RNA polymerase and containing no protein translational start site, said promoter being inserted upstream from a protein ATG translational start site of said gene, at a position to obtain production of said protein, to form a fused gene having said Shine-Dalgarno sequence, said transcription initiation site, and the ATG signalling the start point of translation, inserting said plasmid into said bacteria to transform said bacteria with said plasmid containing said fused gene, and culturing the transformed bacteria to produce said unfused prokaryotic or eukaryotic protein.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 59 U.S. patent(s) that reference this one
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