 |
|
|
|
|
|
 Title: |
US4650764:
Helper cell
[ Derwent Title ]
|
| Country: |
US United States of America
|
| |
| Inventor: |
Temin, Howard M.; Madison, WI
Watanabe, Shinichi; Madison, WI
|
| Assignee: |
Wisconsin Alumni Research Foundation, Madison, WI
other patents from WISCONSIN ALUMNI RESEARCH FOUNDATION (631280) (approx. 1,178)
News, Profiles, Stocks and More about this company
|
| Published / Filed: |
1987-03-17
/ 1984-03-26
|
| Application Number: |
US1984000593175
|
| IPC Code: |
Advanced:
C12N 15/867;
Core:
more...
IPC-7:
C12N 5/00;
C12N 7/04;
C12N 15/00;
C12R 1/91;
|
| U.S. Class: |
Current:
435/350;
435/236;
435/948;
Original:
435/240;
435/172.3;
435/091;
435/236;
435/948;
935/032;
935/071;
935/070;
|
| Field of Search: |
435/068,70,91,172.3,240,236,241,317,948,239
935/032,34,57,58,70,71,63,62
|
| Government Interest: |
This invention was made with Government support under NIH Grant Nos. P01 CA 22443, P30 CA0 7175 and T32 CA0 9075 awarded by the Department of Health and Human Services. The Government has certain rights in this invention.
|
| Priority Number: |
| 1983-04-12 |
US1983000484323 |
|
| Abstract: |
A helper cell for providing retrovirus protein which is required by a normally replication incompetent recombinant retrovirus gene sequence in order to replicate is disclosed. In one embodiment there is a eukaryotic host cell, a first retrovirus gene sequence in the cell which has a helper portion coding for a retrovirus protein and which is capable of expressing the retrovirus protein, and a defective portion which renders the gene sequence replication imcompetent. In this embodiment, there is also a second retrovirus gene sequence in the cell having a defective retrovirus port
This invention was made with Government support under NIH Grant Nos. P01 CA 22443, P30 CA0 7175 and T32 CA0 9075 awarded by the Department of Health and Human Services. The Government has certain rights in this invention.
|
| Attorney, Agent or Firm: |
Quarles & Brady ;
|
| Primary / Asst. Examiners: |
Warren, Charles F.; Tarcza, John Edward
|
| INPADOC Legal Status: |
Show legal status actions
|
|
|
|
|
|
|
| Parent Case: |
This application is a continuation in part of application Ser. No. 484,323 filed Apr. 12, 1983, now abandoned.
|
| Family: |
None
|
First Claim:
Show all 5 claims |
We claim:
1. A helper cell, comprising:
- a host cell;
- a first retrovirus helper gene sequence in the cell which has a helper portion coding for a retrovirus virion protein and which is capable of expressing the virion protein, and a defective encapsidation portion which renders the cell by itself unable to form the infectious virus which the first retrovirus helper gene sequence codes for;
- a second retrovirus helped gene sequence in the cell having a defective helped portion which would have rendered the cell unable to form the infectious virus which the second retrovirus helped gene sequence codes for if suitable virion protein had not been supplied from expression of the first retrovirus helper portion or from another source; and
- said second retrovirus helped gene sequence being a recombinant retrovirus sequence with a first part that is retrovirus and a second part that is a foreign gene protein coding part;
- whereby the virion protein expressed by the first helper portion can permit the second retrovirus helped gene sequence to form infectious recombinant virus coding for the foreign gene.
|
| Background / Summary: |
Show background / summary
|
| Drawing Descriptions: |
Show drawing descriptions
|
| Description: |
Show description
|
| Forward References: |
Show 118 U.S. patent(s) that reference this one
|
|
|
|
|
|
|
| Foreign References: |
None
|
| Other References: |
Tabin et al, "Adaptation of a Retrovirus as a Eukaryotic Vector Transmitting the HSV Thymidine Kinase Gene", Molecular and Cellular Biology, 2(4) pp. 426-436 (4-1982).
(11 pages)
Cited by 33 patents
Wei et al, "Construction and Isolation of a Transmissible Retrovirus Containing the Src Gene of Harvey Murine Sarcoma Virus", Journal of Virology, 39(3) pp. 935-944 (1981).
(10 pages)
Cited by 29 patents
Steimer et al, "Envelope Assembly Mutant of Rouse Sarcoma Virus", Journal of Virology, 36(3), pp. 883-888 (1980).
(6 pages)
Robbins et al, "Molecular Cloning of Integrated Simian Sarcoma Virus: Organization of Infectious DNA Clones", Proceedings of the National Academy of Sciences, 78(5) pp. 2918-2922 (1981).
(5 pages)
O'Rear et al, "Mapping of Alterations in Noninfectious Proviruses of Spleen Necrosis Virus", Journal of Virology, 39(1) pp. 138-149 (1981).
(12 pages)
Linial, "Transfer of Defective Avian Tumor Virus Genomes by Rous Sarcoma Virus RNA Packaging Mutant", Journal of Virology, 38(1) pp. 380-382 (1981).
Watanabe et al, "Encapsidation Sequence Required for Retrovirus Vectors", Eukaryotic Viral Vectors ed. Gluzman, Cold Spring Harbor Lab. 1982 p. 115.
Article by K. Shimotohno & H. Temin, "Formation of Infectious Progeny Virus After Insertion of Herpes Simplex Thymidine Kinase Gene Into DNA of an Avian Retrovirus", 26 Cell 67-77 (1981).
(11 pages)
Cited by 28 patents
Article by H. Temin, "Structure, Variation and Synthesis of Retrovirus Long Terminal Repeat", 27 Cell 1-3 (1981).
(3 pages)
Cited by 2 patents
Article by K. Shimotohno & H. Temin, "Loss of Intervening Sequences in Genomic Mouse Alpha Globin DNA Inserted in an Infectious Retrovirus Vector", 299 Nature 265-268 (1982).
(4 pages)
Article by S. Watanabe & H. Temin, "Encapsidation Sequences for Spleen Necrosis Virus, An Avian Retrovirus, are Between the 5' Long Terminal Repeat and the Start of the Gag Gene", 79 Proc. Nat'l. Acad. Sci., USA 5986-5990 (1982).
(5 pages)
Cited by 16 patents
Article by J. O'Rear et al, "Infectious and Noninfectious Recombinant Clones of the Provirus of SNV Differ in Cellular DNA and are Apparently the Same in Viral DNA", 20 Cell 423-430 (1980).
(8 pages)
Cited by 2 patents
Article by I. Chen et al, "Characterization of Reticuleondotheliosis Virus Stain T DNA and Isolation of a Novel Variant of Reticuleondotheliosis Virus Strain T by Molecular Cloning", 40 J. Virol 800-811 (1981).
(12 pages)
Cited by 4 patents
Article by P. Southern and P. Berg, "Mammalian Cell Transformation with SV40 Hybrid Plasmid Vectors", Eukaryotic Viral Vectors, pp. 41-45 (Cold Spring Harbor Laboratory 1982).
Article by J. O'Rear and H. Temin, "Spontaneous Changes in Nucleotide Sequence in Provirus of Spleen Necrosis Virus, an Avian Retrovirus", 79 Proc. Nat'l. Acad. Sci., USA pp. 1230-1234 (1982).
Article by Y. Gluzman, "SV40 Transformed Simian Cells Support the Replication of Early SV40 Mutants", 23 Cell 175-182 (1981).
|
|
 Nominate this for the Gallery...
|
|
