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 Title: |
US5124041:
Biomolecule sample immobilization
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Sheer, Donald G.; Foster City, CA
Kochersperger, Michael L.; Menlo Park, CA
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| Assignee: |
Applied Biosystems, Inc., Foster City, CA
other patents from APPLIED BIOSYSTEMS, INC. (33180) (approx. 66)
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| Published / Filed: |
1992-06-23
/ 1991-05-01
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| Application Number: |
US1991000697916
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| IPC Code: |
Advanced:
B01D 15/00;
B01D 61/00;
B01D 61/18;
B01L 3/00;
C07K 1/36;
Core:
C07K 1/00;
more...
IPC-7:
B01D 15/08;
B01D 61/14;
B01D 69/12;
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| U.S. Class: |
Current:
210/641;
210/321.64;
210/321.67;
210/321.84;
210/359;
210/514;
210/650;
210/651;
210/691;
210/781;
210/782;
210/806;
422/056;
422/060;
422/061;
422/072;
422/101;
435/006;
436/170;
436/178;
436/501;
530/415;
Original:
210/641;
210/650;
210/651;
210/691;
210/781;
210/782;
210/806;
210/321.64;
210/321.67;
210/321.84;
210/359;
210/502.1;
210/514;
422/056;
422/060;
422/061;
422/072;
422/101;
435/006;
436/501;
436/170;
436/178;
530/415;
935/077;
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| Field of Search: |
210/641,650,651,690,691,781,782,787,789,321.64,321.67,321.84,359,502.1,514,515,806
422/056,58,60,61,72,100,101,102
435/006
436/165,169,170,501,177,178,180
530/412,413,415,427
935/009,77,78
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| Priority Number: |
| 1989-07-28 |
US1989000386847 |
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| Abstract: |
A filter system for separating and binding biomolecules contained in a solution includes a first filter means for selectively passing components of the solution that are smaller than the biomolecules, thereby concentrating the biomolecules in the solution. Also included is a binding membrane means having an affinity for the biomolecules for binding the biomolecules to the binding membrane means, with the binding membrane means being located where the biomolecules are concentrated by the first filter means. In the preferred mode, the binding membrane means is chosen to selectively bind the biomolecule of interest. According to a preferred method of the invention, biomolecules from a sample volume are bound to a binding membrane by urging the sample volume against a biomolecule-binding support membrane that is in contact with a cut-off filter membrane such that the sample volume will pass through the biomolecule-binding support membrane before passing through the cut-off filter membrane. The concept is to concentrate the biomolecules of interest by means of the cut-off membrane in the vicinity of the binding membrane, thereby increasing the binding yield on the binding membrane. In the preferred mode, the sample volume is urged against the binding membrane by centrifugation.
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| Attorney, Agent or Firm: |
Smith, Joseph H. ;
Grubman, Ronald E. ;
Boys, Donald R. ;
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| Primary / Asst. Examiners: |
Jones, W. Gary;
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| INPADOC Legal Status: |
Show legal status actions
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