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 Title: |
US5905042:
Cultured inner cell mass cell lines derived from bovine or porcine embryos
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Stice, Steven L.; Belchertown, MA
Golueke, Paul J.; Belchertown, MA
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| Assignee: |
University of Massachusetts, A Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Amherst, MA
other patents from UNIVERSITY OF MASSACHUSETTS AT AMHERST (599695) (approx. 13)
News, Profiles, Stocks and More about this company
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| Published / Filed: |
1999-05-18
/ 1996-04-01
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| Application Number: |
US1996000626054
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| IPC Code: |
Advanced:
A01K 67/027;
C12N 5/06;
C12N 5/10;
C12N 15/09;
C12N 15/87;
Core:
more...
IPC-7:
A01K 67//027;
C12N 5/06;
C12N 15/85;
C12N 15/86;
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| ECLA Code: |
A01K67/027M; C12N5/06B2; C12N5/06B2P; C12N15/877B; M12N501/235; M12N502/16; M12N510/00; K01K217/05;
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| U.S. Class: |
Current:
435/373;
435/325;
435/378;
435/379;
435/380;
435/381;
800/015;
800/017;
800/021;
Original:
435/373;
435/325;
435/378;
435/379;
435/380;
435/381;
800/015;
800/017;
800/021;
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| Field of Search: |
435/006,7.21,172.1,172.3,240.2,240.21,325,373,378,404,379,380,381
425/9.1
935/070
800/002,15,17,21
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| Priority Number: |
| 1996-04-01 |
US1996000626054 |
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| Abstract: |
Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNAs may be introduced into the subject CICM cells and cell lines to produce transgenic cells useful for the production of transgenic embryos, fetuses and/or offspring, e.g., by nuclear transfer procedures.
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| Attorney, Agent or Firm: |
Burns, Doane, Swecker & Mathis, L.L.P. ;
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| Primary / Asst. Examiners: |
Stanton, Brian R.; Wilson, Michael C.
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| INPADOC Legal Status: |
Show legal status actions
Family Legal Status Report
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| Designated Country: |
AL AM AP AZ BA BB BG BY CA CU CZ EE GE HU IS KE KG KP KR KZ LC AT BE CH DE DK ES FI FR GB GR IE IT LI
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| Family: |
Show 23 known family members
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First Claim:
Show all 14 claims |
What is claimed is:
1. A method for producing a bovine or porcine cultured inner cell mass (CICM) cell which may be incorporated into a bovine or porcine embryo to form a chimeric bovine or porcine which consists essentially of the following steps:
- (i) obtaining inner cell mass (ICM) cells from a bovine or porcine blastocyst or ICM progenitor cells from a bovine or porcine preblastocyst stage embryo;
- (ii) culturing said ICM or ICM progenitor cells on a feeder layer culture to produce multilayer cell colonies;
- (iii) identifying from among the cells contained in the cultured ICM or ICM progenitor cell colony those cells which exhibit the following properties:
- (a) small cytoplasmic/nuclear volume ratio ranging from about 10/90 to 50/50;
- (b) cytoplasmic vesicles;
- (c) small individual cells ranging from about 10 to 20 µm in diameter;
- (iv) separating one or a cluster of said identified cells from the rest of the cell colony; and
- (v) passaging said separated ICM or ICM progenitor cells onto another feeder layer culture and maintaining physical contact between the feeder cell layer and the separated cell or cell cluster throughout the entire culturing period to produce CICM cells that may be incorporated into a bovine or porcine embryo to form a chimeric bovine or porcine.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 8 U.S. patent(s) that reference this one
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| Foreign References: |
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| Other Abstract Info: |
CHEMABS 127(23)316566W
CHEMABS 132(01)000951E
DERABS C1997-535447
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| Other References: |
Stice et al., "Pluripotent Bovine Embryonic Cell Lines Direct Embryonic Dlopment Following Nuclear Transfer", Biology of Reproduction, vol. 54, No. 1, Jan. 1996, pp. 100-110.
(11 pages)
Cited by 26 patents
[ISI abstract]
Cibelli J.B. et al., "Production of germline chimeric bovine fetuses from transgenic enbryonic stem cells", Theriogenology, vol. 47, No. 1, Jan. 1997, p. 241.
First et al, Systems for Production of Calves from Cultured Bovine Embryonic Cells, Reproduction, Fertility, and Development, vol. 6, pp. 553-562 (1994).
(10 pages)
Cited by 10 patents
[ISI abstract]
Bartlett et al, Evaluation of extracellular matrices and the plasminogen activator system in sheep inner cell mass and trophectodermal outgrowth in vitro, Biology of Reproduction, vol. 52, pp. 1426-1445 (1995).
Talbot et al, In vitro pluripotency of epiblasts derived from bovine blastocysts, Molecular Reproduction and Development, vol. 42, pp. 35-52 (1995).
(18 pages)
Cited by 2 patents
[ISI abstract]
Talbot et al, Culturing the epiblast cells of the pig blastocyst. In Vitro Cellular and Development Biology, vol. 29A, pp. 543-554 (Jul. 1993).
(12 pages)
Cited by 6 patents
[ISI abstract]
Annelies et al, Isolation and Characterization of Permanent Cell Lines from Inner Cell Mass Cells of Bovine Blastocysts, Molecular Reproduction and Development, vol. 40, pp. 444-454 (1995).
Collas et al, Nuclear Transplantation by Microinjection of Inner Cell Mass and Granulosa Cell Nuclei, Molecular Reproduction and Development, vol. 38, pp. 264-267 (1994).
(4 pages)
Cited by 20 patents
[ISI abstract]
Sims et al, Production of calves by transfer of nuclei from cultured inner cell mass cells, Proceedings of the National Academy of Sciences, USA, vol. 90, pp. 6143-6147 (Jun. 1993).
Seamark, R. et al (1994). Reproduction, Fertility and Development 6, 653-7.
(5 pages)
Cited by 94 patents
[ISI abstract]
Bradley, A. et al (1992). Bio Technology 10, 534-539.
(6 pages)
Cited by 155 patents
[ISI abstract]
Kappel, C. et al (1992). Current Opinion in Biotechnology 3, 548-553.
Sims, M. et al (1994). Proc. Natl. Acad. Sci USA 91, 6143-7.
(5 pages)
Cited by 5 patents
[ISI abstract]
Callard, R. et al (1994). The Cytokine Facts Brdc, Academic Press, pp. 163-167.
Talbot, N. et al (1993). In Vitro Cellular and Developmental Biology 29A, 543-554.
(12 pages)
Cited by 6 patents
[ISI abstract]
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| Continuity Data: |
| Application Number | Filed | Notes |
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US2004000024010 | 2004-12-28 | is a
continuation of |
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US1999000435629
| 1999-11-08 |
(abandoned)
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US1999000435629 | 1999-11-08 | is a
division of |
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US1996000766939
| 1996-12-16 |
(granted)
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US5994619 issued 1999-11-30 Production of chimeric bovine or porcine animals using cultured inner cell mass cells
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US1996000766939 | 1996-12-16 | is a
continuation in part of |
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>US1996000626054<
| 1996-04-01 |
(granted)
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US5905042 issued 1999-05-18 Cultured inner cell mass cell lines derived from bovine or porcine embryos
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