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 Title: |
EP0006694A2:
Method of making a selected protein[German][French]
[ Derwent Title ]
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Country:
Kind: |
EP European Patent Office (EPO)
A2 APPLICATION PUBLISHED WITHOUT SEARCH REPORT i
(See also:
EP0006694A3,
EP0006694B1,
EP0006694B2 )
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| Inventor: |
Gilbert, Walter;
Broome, Stephanie A.;
Villa-Komaroff, Lydia J.;
Efstratiadis, Argiris A.;
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| Assignee: |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE
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| Published / Filed: |
1980-01-09
/ 1979-06-04
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| Application Number: |
EP1979000301054
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| IPC Code: |
Advanced:
C07K 14/005;
C07K 14/195;
C07K 14/41;
C07K 14/575;
C07K 14/62;
C07K 19/00;
C12N 9/86;
C12N 15/00;
C12N 15/09;
C12N 15/66;
C12P 21/00;
C12P 21/02;
C12P 21/04;
C12R 1/19;
IPC-7:
C12N 15/00;
C12P 21/02;
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| ECLA Code: |
C07K14/62; C12N9/86; C12N15/66; M07K319/00; M07K319/034; M07K319/75; M07K319/02;
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| Priority Number: |
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| Abstract: |
A plasmid or phage gene for a periplasmic or extra-cellular bacterial protein is cleaved, a doublestranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.
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| INPADOC Legal Status: |
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| Designated Country: |
AT BE CH DE FR GB IT LU NL SE
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| Family: |
Show 35 known family members
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Description
Expand description |
[From equivalent
EP0006694B2]
+ Technical field of invention
This invention relates to a process for producing specific proteins in bacteria and having them excreted from the bacterial cell and pertains more specifically to inserting the DNA representing the desired non-bacterial protein or part of a protein by recombinant techniques into the plasmid or phage gene for either a periplasmic or an extracellular protein, hereinafter called a "carried protein", transforming a bacterial host with the recombined gene, and culturing the transformed host to excrete the protein. The protein thus produced can be collected by conventional procedures from the culture medium or from the periplasmic space depending upon the choice of carrier protein gene.
+ Background art
+ Disclosure of invention
+ Brief description of the drawings
+ Best mode for carrying out the invention
+ Example
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