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 Title: |
EP0009930A1:
Recombinant DNA, method for preparing it and production of foreign proteins by unicellular hosts containing it[German][French]
[ Derwent Title ]
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Country:
Kind: |
EP European Patent Office (EPO)
A1 APPLICATION PUBLISHED WITH SEARCH REPORT i
(See also:
EP0009930B1,
EP0009930B2 )
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| Inventor: |
Nunber, Jack H.;
Chang, Annie C.Y.;
Cohen, Stanley N.;
Schimke, Robert T.;
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| Assignee: |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
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| Published / Filed: |
1980-04-16
/ 1979-09-27
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| Application Number: |
EP1979000302019
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| IPC Code: |
Advanced:
C12N 9/06;
C12N 15/00;
C12N 15/09;
C12P 19/34;
C12P 21/00;
IPC-7:
C07H 21/04;
C12N 9/02;
C12N 15/00;
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| ECLA Code: |
C12Y105/01003; C12N9/00P8D1C; C12N15/00;
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| Priority Number: |
| 1978-10-10 |
US1978000950100 |
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| Abstract: |
Method and compositions are provided for producing proteins expressed by a DNA sequence free of proteins expressed by other DNA sequences where the DNA sequences comprise a functional recombinant DNA structure. The functional recombinant DNA structure is formed by annealing and/or ligating a first DNA sequence from a first source which encodes for a polypeptide; a second DNA sequence comprising a vector compatible with a unicellular organism which does not exchange genetic information with said first source, said vector having an intact replicon and a promoter for said first DNA sequence; and a third DNA sequence synthetically introduced between said first and second sequences either prior to or concomitantly with annealing and/or ligating of said first and second DNA sequences encoding for a ribosomal binding site of said host, said third DNA sequence positioned adjacent a deoxyribonucleotide triplet encoding for methionine. The recombinant DNA structure is then employed in the transformation of a unicellular host to provide the host the capability of producing the polypeptide free of other polypeptides.
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| INPADOC Legal Status: |
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Family Legal Status Report
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| Designated Country: |
BE CH DE FR GB IT LU NL SE
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| Family: |
Show 7 known family members
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