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 Title: |
EP0508472A2:
Method of producing hybridomas[German][French]
[ Derwent Title ]
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Country:
Kind: |
EP European Patent Office (EPO)
A2 APPLICATION PUBLISHED WITHOUT SEARCH REPORT i
(See also:
EP0508472A3,
EP0508472B1 )
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| Inventor: |
Fujiwara, Katsumi;
Shitara, Kenya;
Ohta, So;
Hanai, Nobuo;
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| Assignee: |
KYOWA HAKKO KOGYO CO., LTD.
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| Published / Filed: |
1992-10-14
/ 1992-04-10
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| Application Number: |
EP1992000106282
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| IPC Code: |
Advanced:
C07K 16/30;
C12N 5/16;
A61K 38/00;
Core:
C07K 16/18;
more...
IPC-7:
A61K 39/00;
C12N 5/12;
C12P 21/08;
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| Priority Number: |
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| Abstract: |
Hybridomas producing a monoclonal antibody of the IgG class are produced by cultivating a hybridoma producing a monoclonal antibody belonging to the IgM class in the presence of an antigen reactive with the IgM antibody, and in the presence of thymocytes. This hybridoma production method makes it easy to obtain monoclonal antibodies of the IgG class to a glycolipid or the like to which monoclonal antibodies of the IgG class are difficult to obtain.
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| INPADOC Legal Status: |
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Family Legal Status Report
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| Designated Country: |
DE FR GB IT
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| Family: |
Show 8 known family members
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Description
Expand description |
[From equivalent
EP0508472A3]
+ FIELD OF THE INVENTION
+ FIELD OF THE INVENTION
This invention relates to a method of producing hybridomas which comprises converting (class-switching) a hybridoma capable of producing a monoclonal antibody belonging to the IgM class to a hybridoma capable of producing a monoclonal antibody belonging to the IgG class. The monoclonal antibodies of the IgG class as produced by said hybridomas can be used in the treatment of various diseases more widely as compared with monoclonal antibodies of the IgM class.
This invention relates to a method of producing hybridomas which comprises converting (class-switching) a hybridoma capable of producing a monoclonal antibody belonging to the IgM class to a hybridoma capable of producing a monoclonal antibody belonging to the IgG class. The monoclonal antibodies of the IgG class as produced by said hybridomas can be used in the treatment of various diseases more widely as compared with monoclonal antibodies of the IgM class.
+ BACKGROUND OF THE INVENTION
+ BACKGROUND OF THE INVENTION
+ SUMMARY OF THE INVENTION
+ SUMMARY OF THE INVENTION
+ Brief Description of the Drawings
+ Brief Description of the Drawings
+ DETAILED DESCRIPTION OF THE INVENTION
+ DETAILED DESCRIPTION OF THE INVENTION
+ (1) Stimulation of hybridomas with an antigen
+ (1) Stimulation of hybridomas with an antigen
+ (2) Isolation of hybridomas resulting from class switching
+ (2) Isolation of hybridomas resulting from class switching
+ (3) Cloning
+ (3) Cloning
+ (4) Preparation of monoclonal antibodies
+ (4) Preparation of monoclonal antibodies
+ (5) Investigation of the activity and reaction specificity of the monoclonal antibodies
+ (5) Investigation of the activity and reaction specificity of the monoclonal antibodies
+ EXAMPLE
+ EXAMPLE
+ (1) Hybridoma selection
+ (1) Hybridoma selection
+ (2) Antigen stimulation of hybridomas and isolation by flow cytometry
+ (2) Antigen stimulation of hybridomas and isolation by flow cytometry
+ (3) Cloning
+ (3) Cloning
+ Enzyme immunoassay (ELISA)
+ Enzyme immunoassay (ELISA)
+ (4) Preparation of monoclonal antibodies
+ (4) Preparation of monoclonal antibodies
+ (5) Activity assay of monoclonal antibodies
+ (5) Activity assay of monoclonal antibodies
+ (6) Testing for reaction specificity
+ (6) Testing for reaction specificity
+ (7) Testing for complement dependent cytotoxicity (CDC)
+ (7) Testing for complement dependent cytotoxicity (CDC)
+ (a) Preparation of target cells
+ (a) Preparation of target cells
+ (b) Preparation of complement
+ (b) Preparation of complement
+ (c) Measurement of CDC activity
+ (c) Measurement of CDC activity
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