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Title: US4342832: Method of constructing a replicable cloning vehicle having quasi-synthetic genes
[ Derwent Title ]

Country: US United States of America

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13 pages

 
Inventor: Goeddel, David V.; Burlingame, CA
Heyneker, Herbert L.; Burlingame, CA

Assignee: Genentech, Inc., South San Francisco, CA
other patents from GENENTECH, INC. (218000) (approx. 2,164)
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Published / Filed: 1982-08-03 / 1979-07-05

Application Number: US1979000055126
IPC Code: Advanced: A61K 38/27; C07G 99/00; C07H 21/00; C07H 21/04; C07K 14/00; C07K 14/575; C07K 14/61; C12N 1/20; C12N 1/21; C12N 15/00; C12N 15/09; C12N 15/10; C12N 15/18; C12N 15/62; C12N 15/63; C12N 15/64; C12N 15/66; C12N 15/70; C12N 15/72; C12P 19/34; C12P 21/00; C12P 21/02; C12R 1/125; C12R 1/19; C12R 1/42; C12R 1/46;
Subclass: C12N;
C12P;
IPC-7:
C12N 15/00;

ECLA Code: C12N15/66; C07K14/61; C12N15/10; C12N15/62; C12N15/70; M07K319/00; M07K319/02; M07K319/50; M07K319/75;

U.S. Class: Current: 435/091.41; 435/069.1; 435/069.2; 435/069.4; 435/069.51; 435/069.8; 435/320.1; 930/120;
Original: 435/172; 435/068; 435/070; 435/317; 536/027;

Field of Search: 435/317,172,68,70,71

Priority Number:
1979-07-05  US1979000055126

Abstract:     Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

Attorney, Agent or Firm: Kiley, Thomas D. ;

Primary / Asst. Examiners: Tanenholtz, Alvin E.;

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First Claim:
Show all 12 claims		 We claim:     1. In the method of constructing a replicable cloning vehicle capable, in a microbial organism, of expressing a particular polypeptide of known amino acid sequence wherein a gene coding for the polypeptide is inserted into a cloning vehicle and placed under the control of an expression promoter, the improvement which comprises:
  • (a) obtaining by reverse transcription from messenger RNA a first gene fragment for an expression product other than said polypeptide, which fragment comprises at least a portion of the coding sequence for said polypeptide;
  • (b) where the first fragment comprises protein-encoding codons for amino acid sequences other than those contained in said polypeptide, eliminating the same while retaining at least a substantial portion of said coding sequence, the resulting fragment nevertheless coding for an expression product other than said polypeptide;
the product of step (a) or, where required, step (b) being a fragment encoding less than all of the amino acid sequence of said polypeptide;
  • (c) providing by organic synthesis one or more synthetic non-reverse transcript-gene fragments encoding the remainder of the amino acid sequence of said polypeptide, at least one of said fragments coding for the amino-terminal portion of the polypeptide; and
  • (d) deploying the synthetic gene fragment(s) of step (c) and that produced in step (a) or (b), as the case may be, in a replicable cloning vehicle in proper reading phase relative to one another and under the control of an expression promoter;
whereby a replicable cloning vehicle capable of expressing the amino acid sequence of said polypeptide is formed.

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Forward References: Show 77 U.S. patent(s) that reference this one

       
U.S. References: Go to Result Set: All U.S. references   |  Forward references (77)   |   Backward references (0)   |   Citation Link

       
Foreign References:
Buy
PDF		 Publication Date IPC Code Assignee   Title
Get PDF - 18pp EP0006694 1979-06  C12N 15/09 HARVARD COLLEGE Method of making a selected protein 
Get PDF - 38pp EP0020147 1980-12  C12N 15/00 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA A DNA transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor 


Other Abstract Info: CHEMABS 094(21)170974D

Other References:
  • Technology Review, pp. 12 and 13, Dec. 1976.
  • Martial et al., Science, vol. 205, Aug. 10, 1979.
  • Shine et al., Nature, vol. 285, Jun. 12, 1980, pp. 456-461. (6 pages) Cited by 27 patents
  • The Economist, pp. 87 and 88, Jul. 14, 1979.
  • Time, Jul. 30, 1970, p. 70.
  • Newmark, Nature, vol. 280, pp. 637 and 638, Aug. 23, 1979. (2 pages) Cited by 6 patents
  • Villa-Komaroff et al., Proc. Natl. Acad. Sci., vol. 75, pp. 3727-3731, Aug. 1978. (5 pages) Cited by 96 patents
  • Seeburg et al., Nature, vol. 276, pp. 795-798, Dec. 1978. (4 pages) Cited by 36 patents
  • Itakura et al., Science, vol. 198, pp. 1056-1063, Dec. 1977. (8 pages) Cited by 180 patents
  • Crea et al., Proc. Natl. Acad. Sci., vol. 75, pp. 5765-5769, Dec. 1978. (5 pages) Cited by 45 patents
  • Klenow et al., Proc. Natl. Acad. Sci., vol. 65, pp. 168-175, Jan. 1970. Cited by 15 patents
  • Sutcliffe, Cold Spring Harbor Symposium 43, pp. 70-90 (1978).
  • Curtis et al., Molecular Cloning of Recombinant DNA, by Scott et al., pp. 99-111 (1977).
  • Ullrich et al., Science, vol. 196, pp. 1313-1319, Jun. 1977.
  • Bolivar et al., Gene 2, pp. 95-113 (1977). (19 pages) Cited by 172 patents
  • Goedel et al., Proc. Natl. Acad. Sci., vol. 76, pp. 106-110, Jan. 1979.
  • Chang et al., Nature, vol. 275, pp. 617-624, Oct. 1978. (8 pages) Cited by 120 patents
  • Maxam et al., Proc. Nat. Acad. Sci., vol. 74, pp. 560-564 (Feb. 1977). (5 pages) Cited by 225 patents
  • Kornberg, DNA Synthesis, pp. 87 and 88, pub. by W. H. Freeman & Co., 1974.
  • Razin et al., Proc. Natl. Acad., vol. 75, pp. 4268-4270, Sep. 1978. (3 pages) Cited by 5 patents
  • Wickens et al., The Journal of Biological Chemistry, vol. 253, No. 7, pp. 2483-2495 (1978). (13 pages) Cited by 20 patents


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