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Title: US4601980: Microbial expression of a gene for human growth hormone
[ Derwent Title ]

Country: US United States of America

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13 pages

 
Inventor: Goeddel, David V.; Burlingame, CA
Heyneker, Herbert L.; Burlingame, CA

Assignee: Genentech Inc., South San Francisco, CA
other patents from GENENTECH, INC. (218000) (approx. 2,164)
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Published / Filed: 1986-07-22 / 1982-03-09

Application Number: US1982000356564
IPC Code: Advanced: A61K 38/27; C07G 99/00; C07H 21/00; C07H 21/04; C07K 14/00; C07K 14/575; C07K 14/61; C12N 1/20; C12N 1/21; C12N 15/00; C12N 15/09; C12N 15/10; C12N 15/18; C12N 15/62; C12N 15/63; C12N 15/64; C12N 15/66; C12N 15/70; C12N 15/72; C12P 19/34; C12P 21/00; C12P 21/02; C12R 1/125; C12R 1/19; C12R 1/42; C12R 1/46;
Subclass: C12N;
C12P;
IPC-7:
C12N 15/00;
C12P 21/02;

ECLA Code: C12N15/66; C07K14/61; C12N15/10; C12N15/62; C12N15/70; M07K319/00; M07K319/02; M07K319/50; M07K319/75;

U.S. Class: Current: 435/069.4; 930/120;
Original: 435/070; 435/172.3; 935/009; 935/013;

Field of Search: 435/172,68,317,70,71,172.3

Priority Number:
1982-03-09  US1982000356564
1979-07-05  US1979000055126

Abstract:     Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.

Primary / Asst. Examiners: Tanenholtz, Alvin E.;

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Related Applications: Go to Result Set: 4 patent(s) that list this one as related
Application Number Filed Patent Pub. Date  Title
US1979000055126 1979-07-05       


       
Parent Case:     This application is a division of application Ser. No. 55,126, filed 7/5/79.

Designated Country: AT NL SE  BR DK NO RO SU  NL 

Family: Show 175 known family members
First Claim:
Show all 3 claims		 We claim:     1. A method of producing human growth hormone which comprises:
  • (a) disposing a culture of bacterial transformants comprising plasmids which, in a transformant bacterium, will express a gene for human growth hormone unaccompanied by the leader sequence of human growth hormone or other extraneous protein bound thereto within a fermenter vessel comprising aeration and agitation means in an aqueous, nutriment-containing fermentation broth;
  • (b) growing up the culture under aeration and agitation while supplying additional nutriments as required to maintain vigorous growth;
  • (c) separating the resulting cellular mass from the fermentation broth;
  • (d) lysing the cells to free the contents thereof;
  • (e) separating cellular debris from supernatant; and
  • (f) isolating and purifying human growth hormone contained in the supernatant.
    •


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Forward References: Show 29 U.S. patent(s) that reference this one

       
U.S. References: Go to Result Set: All U.S. references   |  Forward references (29)   |   Backward references (6)   |   Citation Link

Buy
PDF		 Patent  Pub.Date  Inventor Assignee   Title
Get PDF - 35pp US4190495  1980-02 Curtiss, III  Research Corporation Modified microorganisms and method of preparing and using same
Get PDF - 10pp US4237224  1980-12 Cohen  Board of Trustees of the Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
Get PDF - 4pp US4332892  1982-06 Ptashne et al.  President and Fellows of Harvard College Protein synthesis
Get PDF - 13pp US4342832  1982-08 Goeddel  Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
Get PDF - 24pp US4356270  1982-10 Itakura et al.  Genentech, Inc. Recombinant DNA cloning vehicle
Get PDF - 32pp US4363877  1982-12 Goodman et al.  The Regents of the University of California Recombinant DNA transfer vectors
       
Foreign References:
Buy
PDF		 Publication Date IPC Code Assignee   Title
Get PDF - 18pp EP0006694 1980-01  C12N 15/09 HARVARD COLLEGE Method of making a selected protein 
Get PDF - 27pp EP0009930 1980-04  C12N 15/00 THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY Recombinant DNA, method for preparing it and production of foreign proteins by unicellular hosts containing it 
Get PDF - 79pp EP0020251 1980-12  C12N 15/00 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA DNA transfer vector, host transformed with it, vaccine, and their production 
Get PDF - 38pp EP0020147 1980-12  C12N 15/00 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA A DNA transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor 
Get PDF - 43pp EP0026598 1981-04  C12N 15/00 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA DNA transfer vectors for human proinsulin and human pre-proinsulin, microorganisms transformed thereby, and processes involving such substances 


Other Abstract Info: CHEMABS 094(21)170974D DERABS C81-03727D

Other References:
  • Merk Index Ninth edition 7290 (1976).
  • Li et al., J. Am. Chem. Soc., vol. 92, pp. 7608 & 7609 (1970).
  • Roberts et al., Proc. Natl. Acad. Sci. USA, vol. 76, No. 2, pp. 760-764 (Feb. 1979). (5 pages) Cited by 55 patents
  • Martial et al., Science, vol. 205, pp. 602-606, Aug. 10, 1979. (6 pages) Cited by 64 patents
  • Itakura et al., Science, vol. 198, pp. 1056-1063, Dec. 9, 1977. (8 pages) Cited by 180 patents
  • Backman et al., Cell, vol. 13, pp. 65-71 (Jan. 1978). (7 pages) Cited by 40 patents
  • Bolivar et al., Gene 2, pp. 95-113 (1977). (19 pages) Cited by 172 patents
  • Chang et al., Nature vol. 275, pp. 617-624 (Oct. 1978). (8 pages) Cited by 120 patents
  • Crea et al., PNAS, vol. 75, pp. 5765-5769 (Dec. 1978).
  • Curtis et al., Molecular Cloning of Recombinant DNA by Scott et al., pp. 99-111 (1977).
  • Goeddel et al., PNAS 76, pp. 106-110 (1979).
  • Goeddel et al., Nature vol. 281, pp. 544-548 (Oct. 1979). (5 pages) Cited by 195 patents
  • Humphries et al., Nucleic Acids Res., 4(7), pp. 2389-2406 (1977). (18 pages) Cited by 20 patents
  • Klenow et al., PNAS vol. 65, pp. 168-175 (Jan. 1970).
  • Kornberg, DNA Synthesis, pp. 87-88, pub. by W. H. Freeman & Co., (1974).
  • Marians et al., Nature vol. 263, pp. 744-748 (Oct. 1976). (5 pages) Cited by 14 patents
  • Maxam et al., PNAS vol. 74, pp. 560-564 (Feb. 1977).
  • Newmark, Nature, vol. 280, pp. 637-638 (Aug. 23, 1979). (2 pages) Cited by 6 patents
  • Polisky et al., PNAS 73:11, pp. 3900-3904 (1976).
  • Razin et al., PNAS vol. 75, pp. 4268-4270 (Sep. 1978).
  • Seeburg et al., Nature vol. 276, pp. 795-798 (Dec. 1978). (4 pages) Cited by 36 patents
  • Shine et al., Nature vol. 285, pp. 456-461 (Jun. 1980). (6 pages) Cited by 27 patents
  • Sutcliff, Cold Spring Harbor Symposium 43, pp. 70-90 (1978).
  • Ullrich et al., Science vol. 196, pp. 1313-1319 (1977).
  • Villa-Kamaroff et al., PNAS vol. 75, pp. 3727-3731 (Aug. 1978).
  • Wickens et al., The J. of Biological Chemistry vol. 253, No. 7, pp. 2483-2495 (1978). (13 pages) Cited by 20 patents
  • Chemical Abstracts, vol. 88, p. 249, No. 101458c (Apr. 1978).
  • Chemical Abstracts, vol. 87, No. 114458g.
  • The Economist, pp. 87-88, Jul. 14, 1979.
  • Technology Review, pp. 12 and 13, Dec. 1976.
  • Time, Jul. 30, 1970, p. 70.


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