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 Title: |
US5231011:
Segregated folding determinants for small disulfide-rich peptides
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Hillyard, David R.; Holliday, UT
Olivera, Baldomero M.; Salt Lake City, UT
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| Assignee: |
University of Utah, Salt Lake City, UT
other patents from UNIVERSITY OF UTAH (600055) (approx. 249)
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| Published / Filed: |
1993-07-27
/ 1991-04-18
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| Application Number: |
US1991000689693
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| IPC Code: |
Advanced:
C07K 1/113;
C07K 14/435;
C12P 21/06;
Core:
C07K 1/00;
more...
IPC-7:
C07K 7/00;
C07K 15/00;
C12P 21/00;
C12P 21/06;
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| U.S. Class: |
Current:
435/069.7;
435/068.1;
435/069.1;
435/480;
435/488;
530/300;
530/333;
530/334;
530/338;
530/350;
530/857;
Original:
435/069.7;
435/068.1;
435/069.1;
435/172.3;
530/333;
530/300;
530/334;
530/338;
530/857;
530/350;
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| Field of Search: |
530/333,350,300,334,338,857
435/68.1,69.7,69.1,172.3
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| Government Interest: |
This invention was made with government support under Contract No. N00014-88-K-0178 awarded by the Department of the Navy and under Contract No. GM-22737 awarded by the Department of Health and Human Services. The government has certain rights in the invention.
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| Priority Number: |
| 1991-04-18 |
US1991000689693 |
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| Abstract: |
The preparation of small peptides with multiple disulfide bonds is accomplished by forming a prepropeptide with an N-terminal excised region separated from the cysteine-rich peptide by one or more cleavable amino acid residues. The excised region preferably consists of an N-terminal end providing a hydrophobic signal sequence domain having up to approximately 25 amino acids, and an intermediate central propeptide domain having a variable length of between about 5-50 amino acids. The N-terminal excised region serves as a folding template to direct the formation of specific disulfide bonds in the cysteine-rich peptide. The cysteine-rich peptide is cleaved by enzymes releasing the biologically active peptide.
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| Attorney, Agent or Firm: |
Thorpe, North & Western ;
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| Primary / Asst. Examiners: |
Wax, Robert A.; Hendricks, Keith D.
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| Maintenance Status: |
E3 Expired Check current status
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| INPADOC Legal Status: |
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| Family: |
None
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First Claim:
Show all 13 claims |
What is claimed is:
1. A method of forming a mature biologically active cysteine-rich peptide having specific disulfide bonds between cysteine residues providing a consistent folding pattern which comprises the formation, by cloning methods, solution phase synthesis or solid phase synthesis, or a combination of solution phase synthesis and solid phase synthesis, of a prepropeptide consisting of a C-terminal excised region separated from said cysteine-rich peptide by one or more cleavable amino acid residues wherein the sequence of the N-terminal excised region is derived from conotoxin peptides found in Conus venom and serves as a folding template to direct the formation of said specific disulfide bonds in said cysteine-rich peptide, and then cleaving said mature peptide form said prepropeptide.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
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