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 Title: |
US5264371:
Screening method for neuroprotective compounds
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Miljanich, George P.; Redwood City, CA
Bitner, Robert S.; Mountain View, CA
Bowersox, Stephen S.; Menlo Park, CA
Fox, James A.; Palo Alto, CA
Valentino, Karen L.; San Carlos, CA
Yamashiro, Donald H.; San Francisco, CA
Tsubokawa, Makoto; South San Francisco, CA
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| Assignee: |
Neurex Corporation, Menlo Park, CA
other patents from NEUREX CORPORATION (397535) (approx. 15)
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| Published / Filed: |
1993-11-23
/ 1992-03-23
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| Application Number: |
US1992000855269
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| IPC Code: |
Advanced:
C07K 14/435;
A61K 38/00;
Core:
more...
IPC-7:
G01N 33/567;
G01N 33/68;
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| U.S. Class: |
Current:
436/503;
436/086;
Original:
436/503;
436/086;
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| Field of Search: |
514/012,13,14
530/324,325,326
436/503,86
424/2.9
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| Priority Number: |
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| Abstract: |
A partially purified omega conotoxin binding protein is disclosed. The protein, either in partially purified form or in a synaptosomal preparation, is useful in identifying compounds for use in reducing neuronal damage related to an ischemic condition, such as stroke, in a human patient.
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| Attorney, Agent or Firm: |
Stratford, Carol A. ;
Dehlinger, Peter J. ;
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| Primary / Asst. Examiners: |
Russel, Jeffrey E.;
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| INPADOC Legal Status: |
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Family Legal Status Report
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| Parent Case: |
This is a division of application Ser. No. 07/561,766, filed Aug. 2, 1990, now U.S. Pat. No. 5,189,020, which is a continuation-in-part of application Ser. No. 440,094, filed Nov. 22, 1989, now U.S. Pat. No. 5,051,403.
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| Designated Country: |
AU CA EP HU JP KR NO SU
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| Family: |
Show 23 known family members
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| Claim |
It is claimed:
1. A method of screening compounds for use in reducing neuronal damage resulting from ischemic conditions in a human, comprising
- measuring the binding affinity of the compounds being screened by competitive displacement of omega conotoxin MVIIA from an isolated synaptosomal omega-conotoxin binding protein having a molecular weight of about 210 kilodaltons as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and
- selecting a compound if its binding affinity to the binding protein is sufficient to produce a displacement of said omega conotoxin MVIIA, wherein said displacement is at least as great as that of an omega conotoxin selected form the group consisting of MVIIA, MVIIB, GVIA, GVIIA and RVIA omega conotoxins.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 25 U.S. patent(s) that reference this one
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 115(25)270696K
DERABS C91-192969
DERABS C93-085564
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| Other References: |
Bean, B. P., "Classes of Calcium Channels in Vertebrate Cells," Annu. Rev. Physiol. 51:367-384 (1989).
(18 pages)
Cited by 37 patents
Choi, D. W., "Calcium-mediated neurotoxicity: relationship to specific channel types and role in ischemic damage," TINS 11(10):465-469 (1988).
(5 pages)
Cited by 10 patents
Gray, W. R., et al., "Peptide Toxins from Venomous Conus Snails," Ann. Rev. Biochem. 57:665-700 (1988).
(36 pages)
Cited by 11 patents
Hamilton, S. L., and Perez, M., "Toxins that Affect Voltage-Dependent Calcium Channels," Biochem. Pharmacol. 36(20):3325-3329 (1987).
(5 pages)
Cited by 2 patents
Jones, O. T., et al., "Localization and Mobility of ω-Coonotoxin-Sensitive Ca2+ Channels in Hippocampal CA1 Neurons," Science 224:1189-1193 (1989).
(5 pages)
Cited by 4 patents
Marqueze, B., et al., "Characterization of the ω-Conotoxin-Binding Molecule in Rat Brain Synaptosomes and Cultured Neurons," Mol. Pharmacol. 34:87-90 (1988).
(4 pages)
Cited by 3 patents
Olivera, B. M., et al., "Neuronal Calcium Channel Antagonists. Discrimination between Calcium Channel Subtypes Using ω-Conotoxin from Conus magus Venom," Biochem. 26:2086-2090 (1987).
(5 pages)
Cited by 19 patents
Takemura, M., et al., "Distribution of the ω-Conotoxin Receptor in Rat Brain. An Autoradiographic Mapping," Neuroscience 32:405-416 (1989).
(12 pages)
Cited by 2 patents
Tsien, R. W., et al., "Multiple types of neuronal calcium channels and their selective modulation," TINS 11(10):431-438 (1988).
(8 pages)
Cited by 9 patents
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