 |
|
|
|
|
|
 Title: |
US5334509:
Method for detecting intestinal pathogen dientamoeba fragilis
[ Derwent Title ]
|
| Country: |
US United States of America
|
| |
| Inventor: |
Riordan, Neil H.; Wichita, KS 67226
|
| Assignee: |
None
|
| Published / Filed: |
1994-08-02
/ 1992-10-22
|
| Application Number: |
US1992000964874
|
| IPC Code: |
Advanced:
C12Q 1/04;
Core:
more...
IPC-7:
C12Q 1/04;
G01N 33/48;
|
| ECLA Code: |
C12Q1/04;
|
| U.S. Class: |
Current:
435/034;
435/029;
435/040.51;
436/063;
436/164;
436/800;
Original:
435/034;
435/029;
436/063;
436/164;
436/800;
424/003;
424/007.1;
|
| Field of Search: |
436/063,164,800
435/029,34,38,947
424/002,3,7.1
|
| Priority Number: |
|
| Abstract: |
A method and apparatus for producing detectable intestinal parasites. The method includes obtaining an intestinal mucosa sample (e.g. feces) having intestinal parasites, such as Dienamoeba fragilis; and contacting the obtained intestinal mucosa sample with an acridine base compound (e.g. acridine orange and/or acridine yellow, etc.) such that the intestinal parasites become differentially stained and detectable by a human eye when viewed through a fluorescence microscope. The apparatus includes a kit or the like which includes at least one vessel or vial. Preferably, two vials are contained within the kit with one vial having an isotonic salt solution including a salt, such as sodium chloride, potassium phosphate, etc., and the other vial containing an acridine biological staining compound.
|
| Attorney, Agent or Firm: |
Carpenter, John W. ;
|
| Primary / Asst. Examiners: |
Housel, James C.; Wallenhorst, Maureen M.
|
| Maintenance Status: |
E2 Expired Check current status
|
| INPADOC Legal Status: |
Show legal status actions
Family Legal Status Report
|
| Designated Country: |
AT BB BG BR BY CA CH CN CZ DE DK EP ES FI GB GE HU JP KG KP KR KZ LK LU LV MD MG MN MW NL NO NZ OA PL PT RO RU SD SE
|
| Family: |
Show 4 known family members
|
First Claim:
Show all 20 claims |
I claim:
1. A method for determining if Dientamoeba fragilis is present in a fecal material from a human being comprising the steps of:
- (a) obtaining from a human being a fecal sample;
- (b) contacting the obtained fecal sample of step (a) with an acridine solution in a weight ratio ranging from about 1:10 to about 10:1 such that if Dientamoeba fragilis is present in the fecal sample, the Dientamoeba fragilis becomes stained with a first color, and such that if residual fecal material is present in the fecal sample, the residual fecal material becomes stained with a second color, said acridine solution comprising an aqueous solution and from about 0.40 micrograms/milliliter to about 100 micrograms/milliliter of an acridine biological staining compound; and
- (c) viewing through a fluorescence microscope the obtained fecal sample of step (b) after being contacted with the acridine solution of step (b) and detecting and discovering the presence of the first color, signifying the presence of Dientamoeba fragilis in the fecal sample, and further detecting and discovering the presence of the second color, signifying the presence of the residual fecal material in the fecal sample.
|
| Background / Summary: |
Show background / summary
|
| Drawing Descriptions: |
Show drawing descriptions
|
| Description: |
Show description
|
| Forward References: |
Show 1 U.S. patent(s) that reference this one
|
|
|
|
|
|
|
| Foreign References: |
None
|
| Other Abstract Info: |
CHEMABS 124(09)111725P
DERABS C1994-248395
DERABS C1994-248395
DERABS C1996-068190
|
| Other References: |
Grendon et al., Public Health Reports, vol. v106, Issue n3, May-Jun. 1991, p. 322(4).
(4 pages)
[ISI abstract]
Kodak Laboratory & Research Products, catalog No. 53, 1987, p. 255.
Biology of Microorganisms -5th edition, 1988, p. 604.
Diagnostic Parasitology, Clinical Laboratory Manual, 2nd edition, 1979, pp. 99-101 and 105.
Medical Parasitology, A Self-Instructional Text, 1979, pp. 158-159.
Clinical Diagnosis & Management by Laboratory Methods-16th edition, 1979, pp. 1759-1765 and 1769.
|
|
 Nominate this for the Gallery...
|
|
