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 Title: |
US5480613:
Apparatus kit for detecting Dientamoeba fragilis
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Riordan, Neil H.; Wichita, KS 67226
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| Assignee: |
None
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| Published / Filed: |
1996-01-02
/ 1994-05-09
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| Application Number: |
US1994000239731
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| IPC Code: |
Advanced:
C12Q 1/04;
Core:
more...
IPC-7:
C12Q 1/04;
G01N 33/48;
G01N 37/00;
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| ECLA Code: |
C12Q1/04;
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| U.S. Class: |
Current:
422/061;
435/029;
435/030;
435/034;
435/040.51;
435/287.1;
435/288.7;
435/810;
Original:
422/061;
435/029;
435/030;
435/034;
435/810;
435/040.51;
435/288.7;
435/287.1;
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| Field of Search: |
436/063,800
435/004,29,30,34,38,287,291,296,810,947
424/002,3,7.1
422/061
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| Priority Number: |
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| Abstract: |
A method and apparatus for producing detectable intestinal parasites. The method includes obtaining an intestinal mucosa sample (e.g. feces) having intestinal parasites, such as Dientamoeba fragilis; and contacting the obtained intestinal mucosa sample with an acridine base compound (e.g. acridine orange and/or acridine yellow, etc.) such that the intestinal parasites become differentially stained and detectable by a human eye when viewed through a fluorescence microscope. The apparatus includes a kit or the like which includes at least one vessel or vial. Preferably, two vials are contained within the kit with one vial having an isotonic salt solution including a salt, sodium chloride, potassium phosphate, etc., and the other vial containing an acridine biological staining compound.
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| Attorney, Agent or Firm: |
Hovey, Williams, Timmons & Collins ;
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| Primary / Asst. Examiners: |
Snay, Jeffrey R.; Wallenhorst, Maureen M.
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| Maintenance Status: |
E2 Expired Check current status
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| INPADOC Legal Status: |
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| Parent Case: |
This application is a divisional of application Ser. No. 07/964,874, filed Oct. 22, 1992 now U.S. Pat. No. 5,334,509, issued Aug. 2, 1994.
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| Designated Country: |
AT BB BG BR BY CA CH CN CZ DE DK EP ES FI GB GE HU JP KG KP KR KZ LK LU LV MD MG MN MW NL NO NZ OA PL PT RO RU SD SE
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| Family: |
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First Claim:
Show all 10 claims |
I claim:
1. An apparatus kit for detecting Dientamoeba fragilis in a sample including feces or intestinal mucosa, comprising:
- a centrifugable sample vessel for holding said sample and having therein at least a quantity of isotonic solution, said vessel presenting converging sidewalls defining a region having sediment containing Dientamoeba fragilis and residual material derived from centrifugation of said sample therein;
- a quantity of a staining agent means comprising an acridine compound for contacting and staining said sediment in said sample vessel, said quantity of staining agent means in said kit being present in a container selected from the group consisting of said sample vessel and a staining agent vessel separate from said sample vessel,
- said sample vessel having a sufficient volume to simultaneously accommodate said isotonic solution, said staining agent means and said sediment therein,
- said quantity of staining agent means causing said Dientamoeba fragilis in said sediment to stain a first color and said residual material of the sediment to stain a second color; and
- means for viewing a portion of said stained sediment through a fluorescent microscope for detecting said first color as an indication of the presence of the Dientamoeba fragilis in said sample.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
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