Totally synthetic affinity reagents (US5498538)


	Parent Case:			This application is a continuation of application Ser. No. 08/013,416 filed Feb. 1, 1993, now abandoned, which in turn is a continuation-in-part of application Ser. No. 07/854,133 filed Mar. 19, 1992, now abandoned, which in turn is a continuation of application Ser. No. 07/480,420 filed Feb. 15, 1990, now abandoned, the entire disclosures of which are incorporated herein by reference.
	Designated Country:			BE CH DK ES FR GB GR IT LI LU NL SE IE MC PT KR
	Family:			Show 31 known family members
	First Claim: Show all 26 claims			What is claimed is: 1. A method for identifying a protein, polypeptide and/or peptide which binds to a ligand of choice, comprising: screening a library of recombinant vectors in which each vector encodes one of a plurality of heterofunctional fusion proteins containing (a) a binding domain encoded by a double stranded oligonucleotide assembled by annealing a first nucleotide sequence of the formula 5' X (NNB)_n J Z 3' with a second nucleotide sequence of the formula 3.Z'O U (NNV)_m Y5' where X and Y are restriction enzyme recognition sites, such that X.noteq.Y; N is A, C, G or T; B is G, T or C; V is G, A or C; n is an integer, such that 10<=n<=100; m is an integer, such that 10<=m<=100; Z and Z' are each a sequence of 6, 9 or 12 nucleotides, such that Z and Z' are complementary to each other; and J is A, C, G, T or nothing; O is A, C, G, T or nothing; and U is G, A, C or nothing; provided, however, if any one of J, O or U is nothing then J, O and U are all nothing, and converting the annealed oligonucleotides to a double stranded oligonucleotide, and (b) an effector domain encoded by an oligonucleotide sequence encoding a protein or peptide that enhances expression or detection of the binding domain, by contacting the plurality of heterofunctional fusion proteins with said ligand of choice under conditions conducive to ligand binding and isolating any fusion prosthesis which bind said ligand.
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	Forward References:			Show 37 U.S. patent(s) that reference this one

U.S. References:

Go to Result Set: All U.S. references | Forward references (37) | Backward references (8) | Citation Link

Patent	Pub.Date	Inventor	Assignee	Title
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US5162504	1992-11	Horoszewicz	Cytogen Corporation	Monoclonal antibodies to a new antigenic marker in epithelial prostatic cells and serum of prostatic cancer patients
US5198346	1993-03	Ladner et al.	Protein Engineering Corp.	Generation and selection of novel DNA-binding proteins and polypeptides
US5223409	1993-06	Ladner et al.	Protein Engineering Corp.	Directed evolution of novel binding proteins

Foreign References:

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