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Title: |
US5625033:
Totally synthetic affinity reagents
[ Derwent Title ]

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Country: |
US United States of America

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Inventor: |
Kay, Brian K.; Chapel Hill, NC
Fowlkes, Dana M.; Chapel Hill, NC

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Assignee: |
The University of North Carolina at Chapel Hill, Chapel Hill, NC
other patents from UNIVERSITY OF NORTH CAROLINA (599780) (approx. 336)
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Published / Filed: |
1997-04-29
/ 1995-06-06

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Application Number: |
US1995000471052

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IPC Code: |
Advanced:
C07K 14/47;
C07K 16/00;
C07K 16/18;
C07K 16/30;
C07K 16/40;
C07K 16/44;
C12N 15/10;
C12N 15/62;
C12N 15/70;
C12P 21/04;
C40B 40/02;
Core:
C07K 14/435;
more...
IPC-7:
C07H 21/02;
C12N 5/00;
C12P 21/04;

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ECLA Code: |
C40B40/02; C07K14/47; C07K16/00; C07K16/18; C07K16/30A; C07K16/30P; C07K16/40; C07K16/44; C12N15/10C1; C12N15/10C4; C12N15/10C8; C12N15/10C12; C12N15/62; C12N15/70;

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U.S. Class: |
Current:
530/324;
435/007.1;
435/007.2;
435/069.1;
435/069.7;
435/252.3;
435/320.1;
530/300;
530/350;
536/023.1;
536/023.5;
Original:
530/324;
530/300;
530/350;
536/023.1;
536/023.5;
435/007.1;
435/007.2;
435/065.1;
435/065.7;
435/172.3;
435/252.3;
435/320.1;

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Field of Search: |
435/7.1,7.2,65.1,65.7,172.3,252.3,320.1
530/300,350,324
536/23.1,23.5

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Priority Number: |
| 1995-06-06 |
US1995000471052 |
| 1993-12-30 |
US1993000176500 |
| 1993-02-01 |
US1993000013416 |
| 1992-03-19 |
US1992000854133 |
| 1990-02-15 |
US1990000480420 |

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Abstract: |
A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.

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Attorney, Agent or Firm: |
Pennie & Edmonds ;

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Primary / Asst. Examiners: |
Walsh, Stephen G.; Sorensen, Ken

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Maintenance Status: |
E3 Expired Check current status

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INPADOC Legal Status: |
Show legal status actions
Family Legal Status Report

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Parent Case: |
This application is a divisional of application Ser. No. 08/176,500, filed Dec. 30, 1993, now U.S. Pat. No. 5,498,538 which is a continuation of application Ser. No. 08/013,416 filed Feb. 1, 1993, now abandoned, which is a continuation-in-part of application Ser. No. 07/854,133 filed Mar. 19, 1992, now abandoned, which in turn is a continuation of application Ser. No. 07/480,420 filed Feb. 15, 1990, now abandoned, the entire disclosures of which are incorporated herein by reference.

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Designated Country: |
BE CH DK ES FR GB GR IT LI LU NL SE IE MC PT KR

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Family: |
Show 31 known family members

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Claim |
What is claimed is:
1. A protein which binds specifically to a metal ion and has an amino acid sequence selected from the group consisting of SEQ ID NOs. 34-63.

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Background / Summary: |
Show background / summary

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Drawing Descriptions: |
Show drawing descriptions

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Description: |
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Forward References: |
Show 4 U.S. patent(s) that reference this one

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Foreign References: |
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Publication |
Date |
IPC Code |
Assignee |
Title |
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C12N 15/00
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THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
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Deoxynucleotide linkers to be attached to a cloned DNA coding sequence |
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GENENTECH INC
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Hybrid receptors, nucleic acid encoding them, their preparation, and their use in determination of ligands and their antagonists and agonists. |
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EP0371820
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C12N 15/09
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SALK INST FOR BIOLOGICAL STUDI
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Novel receptors: their identification, characterization, preparation and use |
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EP0383620A2
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A61K 38/00
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REPLIGEN CORPORATION
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Process for making genes encoding random polymers of amino acids |
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G01N 33/50
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BALLIVET MARC
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METHOD FOR OBTAINING DNA, RNA, PEPTIDES, POLYPEPTIDES OR PROTEINS BY MEANS OF A DNA RECOMBINANT TECHNIQUE |
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GB2183661
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1987-06 |
G01N 33/50
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BALLIVET MARC
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METHOD FOR OBTAINING DNA, RNA, PEPTIDES, POLYPEPTIDES OR PROTEINS BY MEANS OF A DNA RECOMBINANT TECHNIQUE |
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1985-07 |
G01N 33/531
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ANTI-IDIOTYPE ANTIBODIES INDUCED BY SYNTHETIC POLYPEPTIDES |
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WO8600991
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1986-02 |
G01N 33/53
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COMMONWEALTH SERUM LABORATORIES COMMISSION
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METHOD FOR DETERMINING MIMOTOPES |
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WO8605803
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1986-10 |
C12N 15/00
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BALLIVET MARC
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METHOD FOR OBTAINING DNA, RNA, PEPTIDES, POLYPEPTIDES OR PROTEINS BY MEANS OF A DNA RECOMBINANT TECHNIQUE |
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WO8606487
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1986-11 |
G01N 33/53
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COMMONWEALTH SERUM LABORATORIES COMMISSION
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METHOD FOR DETERMINING MIMOTOPES |
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WO8803168
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1988-05 |
C12N 15/00
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SALK INST FOR BIOLOGICAL STUDI
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HORMONE RECEPTOR COMPOSITIONS AND METHODS |
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WO8806630
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1988-09 |
C07K 16/00
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GENEX CORPORATION
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METHOD FOR THE PREPARATION OF BINDING MOLECULES |
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WO9001035
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1990-02 |
A61K 38/00
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THE UNITED STATES OF AMERICA, represented by THE ...
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CYTOTOXIC AGENT AGAINST SPECIFIC VIRUS INFECTION |
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WO9002809
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1990-03 |
C12N 15/00
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PROTEIN ENG CORP
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GENERATION AND SELECTION OF RECOMBINANT VARIED BINDING PROTEINS |
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WO9014430
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1990-11 |
C12N 15/09
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SCRIPPS CLINIC AND RESEARCH FOUNDATION
|
A NEW METHOD FOR TAPPING THE IMMUNOLOGICAL REPERTOIRE |
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WO9105058
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1991-04 |
C12N 15/09
|
KAWASAKI GLENN
|
CELL-FREE SYNTHESIS AND ISOLATION OF NOVEL GENES AND POLYPEPTIDES |
 |
WO9104329
|
1991-04 |
C12N 15/09
|
ABBOTT LAB
|
METHOD OF PRODUCING FUSION PROTEINS |
 |
WO9112328
|
1991-08 |
C07K 19/00
|
FOWLKES, Dana, M.
|
TOTALLY SYNTHETIC AFFINITY REAGENTS |
 |
WO9117271
|
1991-11 |
C07K 14/00
|
AFFYMAX TECH NV
|
RECOMBINANT LIBRARY SCREENING METHODS |
 |
WO9116912
|
1991-11 |
A61K 35/14
|
SCRIPPS CLINIC AND RESEARCH FOUNDATION
|
METAL BINDING PROTEINS |
 |
WO9119818
|
1991-12 |
C12N 1/21
|
AFFYMAX TECH NV
|
PEPTIDE LIBRARY AND SCREENING SYSTEMS |
 |
WO9118980
|
1991-12 |
C07K 14/00
|
CETUS CORP
|
COMPOSITIONS AND METHODS FOR IDENTIFYING BIOLOGICALLY ACTIVE MOLECULES |
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WO9200091
|
1992-01 |
A61K 37/02
|
BIOLIGAND INC
|
RANDOM BIO-OLIGOMER LIBRARY, A METHOD OF SYNTHESIS THEREOF, AND A METHOD OF USE THEREOF |
 |
WO9203641
|
1992-03 |
F02B 3/00
|
BRAEM, Roger
|
DEVICE FOR CLEANING A FUEL SUPPLY SYSTEM |
 |
WO9206191
|
1992-04 |
A61K 8/30
|
GUTERMAN, Sonia, Kosow
|
PROTEINACEOUS ANTI-DENTAL PLAQUE AGENTS |
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WO9206204
|
1992-04 |
C07K 14/00
|
IXSYS, INC.
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SURFACE EXPRESSION LIBRARIES OF HETEROMERIC RECEPTORS |
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WO9206176
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1992-04 |
C12N 1/21
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IXSYS INC
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SURFACE EXPRESSION LIBRARIES OF RANDOMIZED PEPTIDES |
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WO9211272
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1992-07 |
C07H 15/12
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IXSYS, INC.
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OPTIMIZATION OF BINDING PROTEINS |
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WO9215679
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1992-09 |
A61K 38/00
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PROTEIN ENGINEERING CORPORATION
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IMPROVED EPITODE DISPLAYING PHAGE |
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WO9215702
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1992-09 |
C07K 16/00
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BRADBURY, Andrew, Raymon, Morton
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THE BIOLOGICAL SELECTION OF USEFUL MOLECULES |
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WO9215677
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1992-09 |
A61K 38/00
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PROTEIN ENGINEERING CORPORATION
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PROCESS FOR THE DEVELOPMENT OF BINDING MINI-PROTEINS |

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Other Abstract Info: |
CHEMABS 115(19)200369G
CHEMABS 121(23)273845T
CHEMABS 129(02)013206D
DERABS C1991-267139
DERABS C1994-279739

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