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 Title: |
US5679565:
Method of preserving pancreatic islets
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Mullen, Yoko; Sherman Oaks, CA
Kenmochi, Takashi; Hanamigawaku, Japan
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1997-10-21
/ 1995-04-10
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| Application Number: |
US1995000420005
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| IPC Code: |
Advanced:
A01N 1/02;
C12N 5/06;
Core:
more...
IPC-7:
C12N 5/00;
C12N 5/06;
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| ECLA Code: |
A01N1/02; C12N5/06B22;
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| U.S. Class: |
Current:
435/374;
435/001.1;
435/260;
435/404;
Original:
435/240.1;
435/260;
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| Field of Search: |
210/647
435/1.1,1.2,1.3,2,183,240.1,260
424/697,709
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| Government Interest: |
This invention was made with government support under grant DK20827, awarded by The National Institutes of Health. The United States government has certain rights in this invention.
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| Priority Number: |
| 1995-04-10 |
US1995000420005 |
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| Abstract: |
A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method includes contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold preservative solution to the warm digest, agitating the warm digest/cold preservative solution at a temperature between about 0° and 15° C., to thereby further digest the partially digested pancreas included in the warm digest, to form cold digest and collecting liquid from the cold digest to form isolated islets. The cold preservative solution and a pancreatic islet preservative solution of the present invention include D-mannitol, K-lactobionate and a buffer.
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| Attorney, Agent or Firm: |
Mueting, Raasch, Gebhardt & Schwappach, P.A. ;
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| Primary / Asst. Examiners: |
Kim, John;
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| INPADOC Legal Status: |
Show legal status actions
Family Legal Status Report
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| Family: |
Show 2 known family members
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First Claim:
Show all 12 claims |
What is claimed is:
1. A pancreatic islet preservative solution comprising D-mannitol, at least about 80 mM K-lactobionate and a buffer.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
Show drawing descriptions
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| Description: |
Show description
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| Forward References: |
Show 7 U.S. patent(s) that reference this one
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| Foreign References: |
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| Other Abstract Info: |
CHEMABS 127(22)305045Z
CHEMABS 127(22)305045Z
DERABS C1997-525727
DERABS C1997-525727
DERABS C1999-403962
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| Other References: |
"Automated Method for Isolation of Human Pancreatic Islets," C. Ricordi, P.E. Lacy, E.H. Finke, B.J. Olack, and D.W. Scharp, Diabetes, vol. 37, Apr. 1988, pp. 413-420.
(8 pages)
Cited by 5 patents
"A Comparison of Four Solutions for Cold Storage of Pancreatic Islets," V.D.A. Delfino, D.W.R. Gray, C. K. Leow, S. Shimizu, D.J.P. Ferguson, and P. J. Morris, Transplantation, vol. 56, No. 6, Dec. 1993, pp. 1325-1330.
(6 pages)
Cited by 3 patents
[ISI abstract]
"Improved Yield and Survival of Porcine Islets With Newly Developed Lap-1 Solution," T. Kenmochi, M. Miyamoto, Y. Nakagawa, S. Une, P.Y. Benhamou, and Y. Mullen, Transplantation Proc., vol. 26, No. 6, Dec. 1994, pp. 4012.
"Comparison of Islet Isolation Techniques in Dogs: Over 90% Purified Islets Using UW Solution" M.P.M. van der Burg, H.G. Gooszen, R.J. Ploeg, J.P. Scherft, M.J. Field, O.R. Guicherit, J.L. Terrpstra, F.T. vande Woude, M. Frolich, and J.A. Bruijn, Transplantation Proceedings, vol. 22, No. 2, Apr. 1990, pp. 795-796.
(2 pages)
"Metabolic Control After Autotransplantation of Highly Purified Canine Pancreatic Islets Isolated in UW Solution," M.P.M. vander Burg, O.R. Guicherit, R.J. Ploeg, M. Frolich, J.A. Bruijn, J.P. Scherft, and H.G. Gooszen, Transplantation, vol. 23, No. 1, Feb. 1991, pp. 785-786.
(2 pages)
Cited by 2 patents
[ISI abstract]
P.Y. Benhamou et al., "Ultraviolet Light Irradiation Reduces Human Islet Immunogenicity Without Altering Islet Function," Horm. Metab. Res., 27,113-120 (1995).
(8 pages)
Cited by 2 patents
[ISI abstract]
P.Y. Benhamou et al., "Human Islet Isolation in 104 Consecutive Cases," Transplantation, 57(12), 1804-1810 (1994).
(7 pages)
Cited by 2 patents
[ISI abstract]
Gray et al., "The Use of Fluorescein Diacetate and Ethidium Bromide as a Viability Stain for Isolated Islets of Langerhans," Stain Technology, 62(6), 373-381 (1987).
M.P.M. van der Burg et al., "Pancreatic Islet Isolation with UW Solution: A New Concept," Transplantation Proceedings, 22(4), 2050-2051 (1990).
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