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 Title: |
US5681711:
Method for promoting apoptosis in mammalian neual cells
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Bredesen, Dale E.; Rancho Santa Fe, CA
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| Assignee: |
University of California-Los Angeles, Los Angeles, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1997-10-28
/ 1995-03-27
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| Application Number: |
US1995000411011
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| IPC Code: |
Advanced:
G01N 33/50;
Core:
more...
IPC-7:
C12N 5/08;
C12N 15/09;
C12Q 1/02;
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| ECLA Code: |
G01N33/50D2F4; G01N33/50D2; S01N510/00;
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| U.S. Class: |
Current:
435/029;
435/368;
Original:
435/029;
435/172.3;
435/368;
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| Field of Search: |
435/69.1,240.2,29,172.3
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| Priority Number: |
| 1995-03-27 |
US1995000411011 |
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| Abstract: |
A method for determining whether a compound inhibits apoptosis induction in neural cells expressing a FALS-associated sod-1 mutant. A neural cell culture is provided having a plurality of neural cells expressing a FALS-associated sod-1 mutant, wherein through the expression of the sod-1 mutant, the neural cells have a low resistance to apoptosis induction. The neural cell culture is treated with a substance to form a modified cell culture and apoptosis is induced in the modified cell culture. The modified cell culture is then assayed to determine whether it has a higher resistance to apoptosis induction than the untreated neural cell culture. The method is useful in screening substances for use as potential drugs for treating ALS.
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| Attorney, Agent or Firm: |
Gray Cary Ware & Freidenrich ;
Reiter, Stephen E. ;
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| Primary / Asst. Examiners: |
Elliott, George G.; Nelson, Amy J.
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| INPADOC Legal Status: |
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| Designated Country: |
AL AM AP AT AZ BB BG BR BY CA CH CN CZ DE DK EE EP ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN
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| Family: |
Show 3 known family members
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First Claim:
Show all 5 claims |
What is claimed is:
1. A method for screening a substance for potential efficacy as a drug for treating amytropic lateral sclerosis (ALS), said method comprising the steps of:
- providing an untreated ALS cell culture model comprising a plurality of neural cells expressing a sod-1 mutant, wherein through the expression of said sod-1 mutant, said untreated ALS cell culture model has a low resistance to apoptosis induction;
- treating said ALS cell culture model with a substance to form a treated ALS cell culture model;
- inducing apoptosis in said treated ALS cell culture model;
- determining whether said treated ALS cell culture model has a higher resistance to apoptosis induction than said untreated ALS cell culture model; and
- identifying said substance as having potential efficacy as a drug for treating ALS if said treated cell culture model is determined to have higher resistance to apoptosis induction than said untreated ALS cell culture model.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 5 U.S. patent(s) that reference this one
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