Bone and prostate-derived protein factors affecting prostate cancer growth, differentiation, and metastasis (US5728815)


	Parent Case:			BACKGROUND OF THE INVENTION The present application is a continuation-in-part of U.S. patent application Ser. No. 07/859,228 filed Mar. 30, 1992, now abandoned, the entire text of which is herein incorporated by reference without disclaimer.
	Designated Country:			AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE AU CA EP JP
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	First Claim: Show all 15 claims			What is claimed is: 1. An isolated human prostate cell growth factor which stimulates prostate cell growth, the factor being obtained by a process that comprises the steps of: (a) culturing human bone stromal cells in serum-conditioned media to produce conditioned media; (b) passing said conditioned media over a heparin-affinity column in a low salt-containing buffer to bind the growth factor to the column; (c) washing the column to remove one or more contaminants; (d) eluting bound growth factor from the column with a high salt buffer; (e) fractionating according to size; and (f) further purifying by subjecting said factor to non-reducing SDS-PAGE gel electrophoresis, and electroeluting the protein band having an apparent molecular weight of about 220 kD in said SDS-PAGE gel; and wherein said protein stimulates prostate cell growth.
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	Forward References:			Show 5 U.S. patent(s) that reference this one


	U.S. References:			Go to Result Set: All U.S. references \| Forward references (5) \| Backward references (0) \| Citation Link


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	Other Abstract Info:			CHEMABS 123(15)196045U CHEMABS 128(19)226688D CHEMABS 127(25)342252K CHEMABS 128(19)226688D DERABS C1995-263651 DERABS C1997-525742
	Other References:			Scopes 1982 Protein Purification -- Principles and Practice Springer-Verlag NY Section 4.5. Hauschka et al. 1986. J. Biol. Chem. 261:12665-12674 Li, et al. 1993. J. Urol. 149 (4 Supple) 481A. M. Chackal-Roy and B.R. Zetter, Faseb Journal, 4(7): A1989, 1990, Abstract No. 1723. M. Chackal-Roy et al., "Stimulation of Human Prostatic Carcinoma Cell Growth by Factors Present in Human Bone Marrow," The American Society for Clinical Investigation, Inc., 84:43-50, 1989. (8 pages) Cited by 5 patents Leland, W.K. Chung, "Fibroblasts Are Critical Determinants in Prostatic Cancer Growth and Dissemination," Cancer and Metastasis Reviews, 10:263-274, 1991. (12 pages) Cited by 2 patents [ISI abstract] Leland, W.K. Chung et al., "Reciprocal Mesenchymal-Epithelial Interaction Affecting Prostate Tumor Growth an Hormonal Responsiveness," Cancer Surveys, 11:91-121, 1991. (31 pages) Cited by 2 patents [ISI abstract] Martin Gleave et al., "Acceleration of Human Prostate Cancer Growth in Vivo by Factors Produced by Prostate and Bond Fibroblasts," Cancer Research, 51:3753-3761, 1991. (9 pages) Cited by 6 patents [ISI abstract] Martin Gleave et al., Journal of Urology, 145 (4 Suppl.), 213A, 1991, Abstract No. 1. M.E. Gleave et al., "Prostate and Bone Fibroblasts Induce Human Prostate Cancer Growth in Vivo: Implications for Bidirectional Tumor-Stromal Cell Interaction in Prostate Carcinoma Growth and Metastasis," Journal of Urology, 147:1151-1159, 1992. (9 pages) Cited by 2 patents [ISI abstract] Martin E. Gleave et al., "Serum Prostate Specific Antigen Levels in Mice Bearing Human Prostate LNCaP Tumors Are Determined by Tumor, Volume and Endocrine and Growth Factors," Cancer Research, 52:1598-1605, 1992. (8 pages) Cited by 9 patents [ISI abstract] Zetter, Bruce R. et al., "Selective Stimulation of Prostatic Carcinoma Cell Proliferation by Transferrin," Proceedings of the National Academy of Science, 89:6197-6201, 1992. (5 pages) Cited by 11 patents [ISI abstract] Bruce Alberts et al., "Molecular Biology of The Cell," 2d ed. Ch. 12:682-683. "Treatment of Metastatic Disease," Urologic Cancer, 12:6-7, 1993. Leland W.K. Chung et al., "Human Prostate Cancer Model: Roles of Growth Factors and Extracellular Matrices," Journal of Cellular Biochemistry, Supplement 16H:99-105, 1992. (7 pages) Cited by 2 patents [ISI abstract] Jer-Tsong Hsieh et al., "Autocrine Regulation of Prostate-specific Antigen Expression in a Human Prostatic Cancer (LNCaP) Subline¹," Cancer Research, 53:2852-2857, 1993. (6 pages) [ISI abstract] Leland, W.K. Chung, "Implications of stromal-epithelial interaction in human prostate cancer growth, progression and differentiation," Cancer Biology, 4:183-192,1993. (10 pages) Cited by 2 patents [ISI abstract] J.T. Hsieh et al., "Characterization of Non-Androgenic Prostate-Specific Autocrine Factor(s) with Stimulatory Effect on PSA Gene Expression in Androgen-Independent Human Prostate Cancer Cells," J. of Urol., 149:222A, 1993. J.T. Hsieh et al., "Detection of a Non-Androgen PSA-Inducing Protein Factor(s) in Bone Marrow Aspirates of Patients with Androgen-Refractory Prostate Tumors: A Potential Prognostic Marker(s) for Disease Progression," J. Urol., 149:257A, 1993. Eamonn Rogers et al., "The Role of PSA in the Treatment of Radiorecurrent Prostatic Adenocarcinoma by Salvage Radical Prostatectomy".
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