 |
|
|
|
|
|
 Title: |
US5776772:
Method for producing secretable glycosyltransferases and other golgi processing enzymes
[ Derwent Title ]
|
| Country: |
US United States of America
|
| |
| Inventor: |
Paulson, James G.; Del Mar, CA
Ujita-Lee, Eryn; Redondo Beach, CA
Colley, Karen J.; Oak Park, IL
Adler, Beverly; Newbury Park, CA
Browne, Jeffrey K.; Camaville, CA
Weinstein, Jasminder; Westlake Village, CA
|
| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
News, Profiles, Stocks and More about this company
|
| Published / Filed: |
1998-07-07
/ 1996-01-30
|
| Application Number: |
US1996000593865
|
| IPC Code: |
Advanced:
C12N 9/10;
C12N 15/54;
C12P 19/18;
C12P 19/26;
Core:
C12P 19/00;
more...
IPC-7:
C12N 15/00;
C12N 15/54;
C12N 15/63;
C12N 15/79;
|
| ECLA Code: |
C12N9/10D1; C12P19/18; C12P19/26;
|
| U.S. Class: |
Current:
435/320.1;
435/041;
435/069.1;
435/070.1;
435/097;
435/100;
435/197;
435/325;
536/023.5;
Original:
435/320.1;
435/041;
435/069.1;
435/070.1;
435/172.3;
435/097;
435/197;
435/100;
435/325;
935/048;
935/060;
935/070;
935/072;
935/047;
536/023.5;
|
| Field of Search: |
435/320.1,41,69.1,70.1,172.3,97,197,100,240.2,325
935/048,60,70,72,47
536/23.5
|
| Priority Number: |
|
| Abstract: |
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
|
| Attorney, Agent or Firm: |
Oppenheimer Poms Smith ;
|
| Primary / Asst. Examiners: |
Stanton, Brian R.; Schmuck, Jill D.
|
| INPADOC Legal Status: |
None
Family Legal Status Report
|
|
|
|
|
|
|
| Parent Case: |
This is a divisional of application Ser. No. 08/209,604, filed on Mar. 10, 1994, now U.S. Pat. No. 5,541,083, which is a continuation of Ser. No. 07/849,045, filed on May 27, 1992, now abandoned, which is the United States National phase of PCT application Serial number US90/06088 filed Oct. 24, 1990 which is a continuation-in-part of Ser. No. 07/426,577, filed Oct. 24, 1989, now U.S. Pat. No. 5,032,517.
|
| Designated Country: |
BE CH FR GB GR IT LI LU NL SE CA
|
| Family: |
Show 18 known family members
|
First Claim:
Show all 5 claims |
What is claimed is:
1. An expression cassette comprising a promoter operably linked to a DNA sequence, said DNA sequence encoding a secretable glycosyltransferase which lacks the membrane anchor and golgi retention signal.
|
| Background / Summary: |
Show background / summary
|
| Drawing Descriptions: |
Show drawing descriptions
|
| Description: |
Show description
|
| Forward References: |
Show 3 U.S. patent(s) that reference this one
|
|
