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 Title: |
US5798220:
Assay for humoral immunity to macromolecules
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Kossovsky, Nir; Los Angeles, CA
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1998-08-25
/ 1995-05-25
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| Application Number: |
US1995000450860
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| IPC Code: |
Advanced:
G01N 33/53;
G01N 33/545;
G01N 33/548;
G01N 33/552;
G01N 33/553;
Core:
G01N 33/544;
G01N 33/551;
more...
IPC-7:
G01N 33/53;
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| U.S. Class: |
Current:
435/013;
435/007.1;
435/007.92;
435/007.95;
436/072;
436/524;
436/525;
436/527;
436/528;
436/531;
Original:
435/013;
435/007.1;
435/007.92;
435/007.95;
436/524;
436/525;
436/527;
436/528;
436/531;
436/072;
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| Field of Search: |
422/057,58
436/506,519,522,524,528,529,530,72,73,809,810,525,531,527
435/7.1,7.92,7.95,13,291,299
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| Priority Number: |
| 1995-05-25 |
US1995000450860 |
| 1993-03-11 |
US1993000029775 |
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| Abstract: |
A method for detecting immunogenic responses to native macromolecules. The native macromolecule is bound to a biomaterial or pharmacologic support surface and used in an immunoassay to screen biological fluids for antibodies to the macromolecule in its bound state. The method is used to screen for immune responses to implanted biomaterials and pharmacologically administered agents where native macromolecules which have interacted with the implant are conformationally altered and elicit an immune response.
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| Attorney, Agent or Firm: |
Oppenheimer Wolff & Donnelly LLP ;
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| Primary / Asst. Examiners: |
Duffy, Patricia;
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| INPADOC Legal Status: |
None
Family Legal Status Report
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| Parent Case: |
This is a continuation of application Ser. No. 08/ 029,775 filed on Mar. 11, 1993 which is now abandoned.
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| Designated Country: |
CA EP JP
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| Family: |
Show 2 known family members
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First Claim:
Show all 5 claims |
What is claimed is:
1. A method for detecting the presence of antibodies to conformationally altered native macromolecules in biological fluid, said method comprising the steps of:
- obtaining a sample of biological fluid from a mammal wherein a biomaterial has been implanted into said mammal, said biomaterial being selected from the group consisting of silicone, stainless steel, polytetraflouroethylene, alumina, zirconia, polyurethane and calcium-phosphate ceramics;
- providing a test surface which is coated with said biomaterial to provide a support surface consisting essentially of said biomaterial;
- wherein said support surface has been treated with a sufficient amount of a native macromolecule for a sufficient time to attach said native macromolecule to said support surface to form conformationally altered native macromolecules which are attached to said test surface, said native macromolecule being selected from the group consisting of fibrinogen, fibronectin, laminin, sphingomyelin and phosphatidylcholine;
- contacting said sample of biological fluid with said conformationally altered native macromolecules for a sufficient time to allow any immunoglobulin which specifically binds with said conformationally altered native macromolecule to bind thereto to form conformationally altered macromolecule/immunoglobulin conjugates;
- detecting the presence of any conformationally altered macromolecule/immunoglobulin conjugates present on said test surface.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
Show drawing descriptions
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| Description: |
Show description
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| Forward References: |
Show 4 U.S. patent(s) that reference this one
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 121(23)278837B
DERABS C94-303200
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| Other References: |
Ronagnoli et al., "Gold-Specific T-Cells in Rheumatoid Arthritis Patients Treated with Gold", Journal of Clinical Investigation, vol. 89, Jan. 1992, pp. 254-258.
Goldblum et al, Aug. 1992. Antibodies to silicone elastomers and reactions to ventriculoperitoneal shunts, Lancet 340:510-13.
(4 pages)
Cited by 2 patents
[ISI abstract]
Goding, 1983, Monoclonal Antibodies : Principles and Practice, Academic Press, Inc., London. pp. 75-80.
Kossovsky, N. and Papasian, N.: "Clinical Reviews: Mammary Implants," Jour.Appl.Biomaterials, vol. 3 (1992) pp. 1-4.
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