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 Title: |
US5800993:
DNA sequencing apparatus and method for a small format gel with a magnified readout
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Bhat, Suraj P.; Los Angeles, CA
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1998-09-01
/ 1996-07-23
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| Application Number: |
US1996000685777
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| IPC Code: |
Advanced:
C12Q 1/68;
Core:
more...
IPC-7:
C12Q 1/68;
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| ECLA Code: |
C12Q1/68E;
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| U.S. Class: |
Current:
435/006;
204/461;
204/466;
Original:
435/006;
204/461;
204/466;
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| Field of Search: |
204/466,467,461
435/006
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| Government Interest: |
This invention was made with Government support under NIH Grant EY06044 awarded by the National Institutes of Health. The Government has certain rights in this invention.
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| Priority Number: |
| 1996-07-23 |
US1996000685777 |
| 1994-07-29 |
US1994000282610 |
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| Abstract: |
The time, difficulty, and expense of running DNA sequencing gels is substantially reduced by running the DNA sequence in a minigel of approximately 8×11 cm at a reduced electrophoretic voltage and without preheating the buffer solution in contact with the gel. The image produced from the sequence gel or autoradiograph is then scanned with a CCD line camera. The pixel map of the autoradiograph image is then magnified using software techniques within the computer so that it is displayed on the CRT screen at an increased scale permitting easy visual separation between the DNA bands.
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| Attorney, Agent or Firm: |
Dawes, Daniel L. ;
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| Primary / Asst. Examiners: |
Houtteman, Scott W.;
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| Parent Case: |
This is a continuation of application Ser. No. 08/282,610 filed on Jul. 29, 1994 now abandoned.
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| Family: |
None
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First Claim:
Show all 21 claims |
I claim:
1. An improvement in a method of DNA sequencing in a gel comprising the steps of:
- providing a minigel cassette characterized by having reduced length and width with the largest dimension thereof being less than approximately 20 cm;
- preparing a DNA sequencing gel in said minigel cassette;
- sequencing a sample of DNA fragments within said DNA sequencing gel in said minigel cassette without prewarming said DNA sequencing gel from room temperature, said step of sequencing being performed at a reduced voltage compared to voltage levels used in large cassettes with the smallest width or length dimension thereof being more than approximately 20 cm to obtain a sequence ladder resolution of a single base pair; and
- producing an image of sequenced DNA fragment bands within said DNA sequencing gel;
- scanning said image with a CCD camera to produce a computer-magnifiable digital image to permit visual identification of said single base pair among said DNA bands without any loss of performance characteristics.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
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