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 Title: |
US5830740:
Serine protease operative between 75°C. and 103°C.
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Miller, Jeffrey H.; Los Angeles, CA
Markiewicz, Peter; Santo Monk, CA
Volkl, Paul; Regensburg, Germany
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| Assignee: |
Research Corporation Technologies, Inc., Tuscson, AZ
other patents from RESEARCH CORPORATION TECHNOLOGIES, INC. (471735) (approx. 327)
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| Published / Filed: |
1998-11-03
/ 1994-07-20
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| Application Number: |
US1994000278042
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| IPC Code: |
Advanced:
C11D 3/386;
C12N 9/52;
C12N 15/57;
Core:
more...
IPC-7:
C11D 3/386;
C12N 9/52;
C12N 15/57;
C12N 15/70;
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| ECLA Code: |
C11D3/386B; C12N9/52;
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| U.S. Class: |
Current:
435/220;
435/068.1;
435/069.1;
435/219;
435/252.3;
435/252.31;
435/252.33;
435/262;
435/320.1;
510/300;
536/023.2;
Original:
435/220;
435/219;
435/068.1;
435/069.1;
435/252.3;
435/252.31;
435/252.33;
435/320.4;
435/262;
510/300;
536/023.2;
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| Field of Search: |
435/219,220,68.1,69.1,252.3,252.31,252.33,262
536/23.2
510/027,300
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| Government Interest: |
This invention was made with Government support under Navy Grant No. N00014-92-J-1403. The Government has certain rights in the invention.
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| Priority Number: |
| 1994-07-20 |
US1994000278042 |
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| Abstract: |
A subtilisin which exhibits proteolytic activity at temperatures ranging from 75 DEG C. to 130 DEG C. The subtilisin has been given the name aerolysin because it was initially isolated from the hyperthermophilic archaeum, Pyrobaculum aerophilum. The amino acid sequence of aerolysin is disclosed as well as the nucleotide sequence which encodes the enzyme. Aerolysin and related modified enzymes are useful as high temperature detergent additives and, among other things, may be used in combination with a wide variety of other detergent agents.
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| Attorney, Agent or Firm: |
Scully, Scott, Murphy & Presser ;
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| Primary / Asst. Examiners: |
Wax, Robert A.; Moore, William W.
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| Maintenance Status: |
CC Certificate of Correction issued
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| INPADOC Legal Status: |
Show legal status actions
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| Family: |
None
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First Claim:
Show all 18 claims |
What is claimed is:
1. An isolated and purified serine protease which exhibits proteolytic activity at temperatures of between about 75° C. and about 130° C., said protease comprising an amino acid sequence as set forth in SEQ ID NO:3 and thermally stable modified sequences thereof.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
Show description
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 129(25)327731P
CHEMABS 129(25)327731P
DERABS C1998-609253
DERABS C1998-609253
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| Other References: |
Arnold, F. H., FASEB Journal, vol. 7, "Engineering proteins for nonnatural environments", pp. 744-749, 1993.
(6 pages)
Cited by 7 patents
[ISI abstract]
Mozhaev, V. V., Trends in Biotechnology, vol. 11, "Mechansm-based strategies for protein thermostablilization", pp. 88-95, 1993.
(8 pages)
[ISI abstract]
Goodenough, P. W., et al., Biochemical Society Transactions, vol. 19, "Protein engineering to change thermal stability for food enzymes", pp. 655-662, 1991.
(8 pages)
Cited by 3 patents
[ISI abstract]
Bell, J. A., et al., in Use of X-Ray Crystallography in the Design of Antiviral Agents, Laver, W. G., and Air, G. M. Eds., Academic Press, Inc., "Approaches to the design of proteins of enhanced thermostability", pp. 233-245, 1990.
Matthews, B. W., et al., Proceedings of the National Academy of Sciences, U.S.A., vol. 84, "Enhanced protein thermostabilityfrom site-directed mutations that decrease the entropy of unfolding", pp. 6663-667, 1987.
(5 pages)
Cited by 11 patents
Volkl, P., et al., Protein Science, vol. 3, "The sequence of a subtilisin-type protease (aerolysin) from the hyperthermophilic archaeum Probaculum aerophilum reveals sites important to thermostability", pp.1329-1340, 1994.
(12 pages)
[ISI abstract]
Hirono, I., et al., Microbial pathogenesis, vol. 13, "Nucleotide sequences and characterization of hemolysin genes from Aeromonas hydrophila and Aeromonas sobria", pp. 433-446, 1992
(14 pages)
[ISI abstract]
Howard, S. P., et al., Journal of Bacteriology, vol. 169, "Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila", pp. 2869-2871, 1987.
(3 pages)
Cited by 2 patents
Volkl et al., Pyrobaculum Aerophilum SP. Nov., A Novel Nitrate-Reducing Hyperthermophilic Archaeum, Applied and Environmental Microbiology, Sep. 1993, pp. 2918-2926.
(9 pages)
[ISI abstract]
Siezen et al., Homology Modelling and Protein Engineering Strategy of Subtilases, the Family of Subtilisin-Like Serine Proteinases, Protein Engineering, vol. 4, No. 7, pp. 719-737, 1991.
(19 pages)
Cited by 53 patents
[ISI abstract]
Meloun et al., Complete Primary Structure of Thermitase From Thermoactinomyces Vulgaris and Its Structural Features Related to the Subtilisin-Type Proteinases, FEBS Letters, vol. 183, No. 2, Apr. 1985, pp. 195-200.
(6 pages)
Cited by 46 patents
Thayer, A.M., Biotechnology's Impact on Chemical Processes Only In Its Infancy, Oct. 14, 1991, C&EN, pp. 13-14.
(2 pages)
[ISI abstract]
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