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 Title: |
US5846749:
Quantitative measurement of tissue protein identified by immunohistochemistry and standardized protein determination
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Slamon, Dennis J.; Woodland Hills, CA
Press, Michael F.; Manhattan Beach, CA
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1998-12-08
/ 1994-10-12
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| Application Number: |
US1994000321688
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| IPC Code: |
Advanced:
G01N 33/50;
Core:
more...
IPC-7:
G01N 33/53;
G01N 33/574;
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| ECLA Code: |
G01N33/50D;
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| U.S. Class: |
Current:
435/007.23;
435/007.21;
435/007.9;
435/007.92;
435/363;
436/063;
436/064;
436/813;
Original:
435/007.23;
435/007.9;
435/007.92;
435/363;
435/007.21;
436/063;
436/064;
436/813;
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| Field of Search: |
435/7.21,7.23,7.9,7.92,240.2,363
436/063,64,813
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| Government Interest: |
This invention was developed at least in part by funding under Grant No. CA36827 and from NCI Grant Nos. CA48780 and CA50589. The government may have an interest in this invention.
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| Priority Number: |
| 1994-10-12 |
US1994000321688 |
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| Abstract: |
Cellular components are quantitated using stained cell samples, computerized image analysis, and cellular standards, where the computerized image analysis value can be translated into the amount of the component per cell. The methodology is demonstrated with breast cancer cells and quantitation of the HER-2/neu gene. The quantitation is shown to have prognostic capability as to the future course of the disease.
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| Attorney, Agent or Firm: |
Trecartin, Richard F. ;
Kresnak, Mark T.Flehr Hohbach Test Albritton and Herbert LLP ;
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| Primary / Asst. Examiners: |
Scheiner, Toni R.;
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| INPADOC Legal Status: |
Show legal status actions
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| Family: |
None
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First Claim:
Show all 12 claims |
What is claimed is:
1. A method for quantitating a surface membrane or cytosol protein, said method comprising:
- (1) staining immobilized fixed cells with a labeled binding composition comprising a probe binding specifically to said surface membrane or cytosol protein, wherein said label can be quantitated by computerized image analysis;
- (2) determining the signal value from a known number of said fixed cells by means of computerized image analysis; and
- determining the amount of said surface membrane or cytosol protein per cell by comparing said signal value obtained from said fixed cells with control values between control cellular compositions having said surface membrane or cytosol protein at different cellular amounts for which signal values are obtained in accordance with steps (1) and (2) and for which the amount of said surface membrane or cytosol protein for a known number of cells is obtained by an alternative method of analysis other than computerized image analysis.
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| Background / Summary: |
Show background / summary
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 41 U.S. patent(s) that reference this one
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 130(05)049514N
CHEMABS 130(05)049514N
DERABS C1999-059052
DERABS C1999-059052
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| Other References: |
Czerniak et al., The Journal of Histochemistry and Cytochemistry, vol. 38, No. 4, pp. 463-466, 1990.
(4 pages)
M.F. Press, et al., "Her-2/neu Expression in Node-negative Breast Cancer: Direct Tissue Quantiation by Computerized Image Analysis and Association of Overexpression with Increased Risk of Recurrent Disease," Cancer Research 53, 4960-4907, Oct. 15, 1993.
(11 pages)
Cited by 15 patents
[ISI abstract]
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