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Title: US5849492: Method for rapid identification of prokaryotic and eukaryotic organisms
[ Derwent Title ]


Country: US United States of America

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18 pages

 
Inventor: Rogan, Peter K.; Lebanon, PA

Assignee: Phylogenetix Laboratories, Inc., Pittsburgh, PA
other patents from PHYLOGENETIX LABORATORIES, INC. (749178) (approx. 1)
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Published / Filed: 1998-12-15 / 1996-10-29

Application Number: US1996000744722

IPC Code: Advanced: C07H 21/00; C12Q 1/68;
Core: more...
IPC-7: C07H 21/02; C12Q 1/68;

ECLA Code: C12Q1/68M; C07H21/00C4; C12Q1/68A8; C12Q1/68M10B; C12Q1/68M10F;

U.S. Class: Current: 435/006; 536/023.1;
Original: 435/006; 536/023.1;

Field of Search: 435/006,91.2 935/077,70 536/23.1

Priority Number:
1996-10-29  US1996000744722
1994-02-28  US1994000203056

Abstract:     A method is described for retrieval of phylogenetically informative DNA sequences which comprise searching for a highly divergent segment of genomic DNA surrounded by two highly conserved segments, designing the universal primers for PCR amplification of the highly divergent region, and amplifying the genomic DNA by PCR technique using universal primers.

Attorney, Agent or Firm: Reed Smith Shaw & McClay LLP ;

Primary / Asst. Examiners: Campbell, Eggerton A.;

INPADOC Legal Status: Show legal status actions

       
Related Applications:
Application Number Filed Patent Pub. Date  Title
US1994000203056 1994-02-28       


       
Parent Case:     This is a continuation of application Ser. No. 08/203,056 filed on Feb. 28, 1994 abandoned.

Family: None

First Claim:
Show all 5 claims
What is claimed is:     1. A method of obtaining data for taxonomic assignment of unknown species comprising:
  • (a) searching for a divergent segment of DNA with low average information content determined quantitiatively surrounded by two conserved segments of said DNA with high average information content determined quantitatively;
  • (b) designing primers for PCR amplification of said divergent segment by constructing a sequence logo for said DNA such that said primers contain a set of sequences present in said sequence logo that encompass the nucleotide variability of said conserved segments, which primers can anneal to said conserved segments;
  • (c) amplifying said divergent segment of DNA by PCR technique using said primers;
  • (d) sequencing the amplified DNA; and
  • (e) comparing the resulting sequences with previously known sequences to determine the most similar related sequences.


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Forward References: Show 6 U.S. patent(s) that reference this one

       
U.S. References: Go to Result Set: All U.S. references   |  Forward references (6)   |   Backward references (0)   |   Citation Link

       
Foreign References: None

Other Abstract Info: CHEMABS 130(05)048293C CHEMABS 130(05)048293C DERABS C1999-094408 DERABS C1999-094408

Other References:
  • Lane et al, PNAS 82:6955-6959, 1985.
  • Bottger, FEMS Microbiol. Letters 65:171-176, 1989. (6 pages) Cited by 7 patents
  • Naito et al, J. Forensic Sciences 37:396-403, 1992. (8 pages) Cited by 2 patents [ISI abstract]
  • Schneider et al, Nucleic Acid Res 18:6097-6100, 1990. (4 pages) Cited by 4 patents


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