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Title: US5854067: Hexokinase inhibitors
[ Derwent Title ]


Country: US United States of America

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Inventor: Newgard, Christopher B.; Dallas, TX
Han, He-Ping; Arlington, TX
Normington, Karl D.; Dallas, TX

Assignee: Board of Regents, The University of texas System, Austin, TX
Betagene, Inc., Dallas, TX
other patents from UNIVERSITY OF TEXAS (600015) (approx. 1,442)
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Published / Filed: 1998-12-29 / 1996-01-19

Application Number: US1996000588983

IPC Code: Advanced: C07K 14/62; C12N 9/10; C12N 9/12; C12N 15/113; C12N 15/54;
IPC-7: C12N 15/00; C12N 15/63; C12N 15/85; C12Q 1/68;

ECLA Code: C12N15/113D; C07K14/62; C12N9/10D1; C12N9/12C; C12Y207/01001; M12N310/121;

U.S. Class: Current: 435/366; 435/004; 435/006; 435/091.1; 435/091.31; 435/183; 435/320.1; 435/325; 536/023.1; 536/024.31; 536/024.5;
Original: 435/366; 425/004; 425/006; 425/091.1; 425/091.31; 425/183; 425/320.1; 425/325; 536/023.1; 536/024.31; 536/024.5;

Field of Search: 435/325,4,6,69.1,320.1,172.3 424/94.1 536/23.1,24.5 514/044 576/24.31

Priority Number:
1996-01-19  US1996000588983

Abstract:     Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their respective genes; hexokinase-specific ribozymes and genes encoding such constructs; and agents that competitively reduce hexokinase activity, e.g., by displacing hexokinase from mitochondria, and their respective genes. The latter group of agents includes inactive hexokinases and fragments thereof that retain mitochondrial binding functions and hexokinase-glucokinase chimeras that further substitute glucokinase activity for hexokinase activity. Mammalian cells including such hexokinase inhibitors, methods of making such cells and various in vitro and in vivo methods of using cells with reduced hexokinase activity are also described herein.

Attorney, Agent or Firm: Arnold, White & Durkee ;

Primary / Asst. Examiners: LeGuyader, John L.; Wang, Andrew

Maintenance Status: E2 Expired  Check current status
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Designated Country: AL AM AP AZ BA BB BG BR BY CA CN CU CZ EE GE HU IL IS JP KE KG KP KR KZ LC  AT BE CH DE DK ES FI FR GB GR IE IT LI 

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First Claim:
Show all 64 claims
What is claimed is:     1. A mammalian host cell comprising an inhibitor of a low Km hexokinase, the inhibitor selected from:
  • (a) a trehalose-6-phosphate synthase enzyme that stimulates the production of trehalose-6-phosphate; or
  • (b) a ribozyme specific for an RNA transcript of a low Km hexokinase gene;
wherein said inhibitor is present in an amount effective to reduce the low Km hexokinase activity of said cell.


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Forward References: Show 30 U.S. patent(s) that reference this one

       
U.S. References: Go to Result Set: All U.S. references   |  Forward references (30)   |   Backward references (5)   |   Citation Link

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Patent  Pub.Date  Inventor Assignee   Title
Get PDF - 5pp US4353888  1982-10 Sefton   Encapsulation of live animal cells
Get PDF - 18pp US5002661  1991-03 Chick et al.  W. R. Grace & Co.-Conn. Artificial pancreatic perfusion device
Get PDF - 14pp US5175085  1992-12 Johnson et al.  Board of Regents, The University of Texas System Methods and compositions for diagnosing autoimmune insulin dependent diabetes mellitus
Get PDF - 34pp US5427940  1995-06 Newgard  Board of Regents, The University of Texas System Engineered cells producing insulin in response to glucose
Get PDF - 16pp US5585479  1996-12 Hoke et al.  The United States of America as represented by the Secretary of the Navy Antisense oligonucleotides directed against human ELAM-I RNA
       
Foreign References:
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Publication Date IPC Code Assignee   Title
Get PDF - 12pp EP0247494A2 1987-12  A01K 67/00 GEN HOSPITAL CORP Transgenic mammal containing heterologous gene 
Get PDF - 155pp WO9500644 1995-05  C07K 14/62 BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM VECTORS FOR GENETICALLY ENGINEERED CELLS THAT PRODUCE INSULIN IN RESPONSE TO GLUCOSE 
Get PDF - 194pp WO9742326 1997-11  C12N 15/54 GODDIJN OSCAR JOHANNES MARIA REGULATING METABOLISM BY MODIFYING THE LEVEL OF TREHALOSE-6-PHOSPHATE 


Other Abstract Info: CHEMABS 127(14)187510X DERABS C1997-385343

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