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Title: US5871947: Method for the determination of low concentrations of heavy metals
[ Derwent Title ]


Country: US United States of America

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13 pages

 
Inventor: Paglia, Donald E.; Sherman Oaks, CA 91403
Renner, Stephen W.; Los Angeles, CA 90049
Bhambhani, Kanta; Bloomfield Hills, MI 48304
Nakatani, Misae; Monterey Park, CA 91754

Assignee: None

Published / Filed: 1999-02-16 / 1997-05-07

Application Number: US1997000852564

IPC Code: Advanced: C12Q 1/34; G01N 33/487;
Core: more...
IPC-7: C12Q 1/00; C12Q 1/34; C12Q 1/42;

ECLA Code: C12Q1/34; G01N33/487B1;

U.S. Class: Current: 435/021; 435/004; 435/018; 436/076; 436/800; 536/026.1; 536/026.72; 536/026.74; 536/026.8; 536/027.8; 536/027.81; 536/028.5; 536/028.53; 556/081;
Original: 435/021; 435/018; 435/004; 536/026.1; 536/028.53; 536/026.8; 536/028.5; 536/027.8; 536/026.74; 536/027.81; 536/026.72; 556/081; 436/076; 436/800;

Field of Search: 435/021,18,4 536/26.1,28.53,26.8,28.5,27.8,26.74,27.81,26.72 556/081 436/076,800

Government Interest:

GOVERNMENT RIGHTS
    This invention was made with U.S. Government support. Accordingly, the U.S. Government has certain rights in this invention.

Priority Number:
1997-05-07  US1997000852564
1996-05-08  US1996000016596P

Abstract: The present invention provides a highly effective method for determining very low concentrations of heavy metals such as lead, cadminum, mercury, and copper in body fluids. Ratios of d-5'-N/Pyr-5'-N activities which display greater deviation and stronger correlation with blood heavy metal concentrations than absolute Pyr-5'-N activities alone, are utilized to provide a highly sensitive, internally controlled biomarker of low-level lead overburden, for example.

Attorney, Agent or Firm: Oppenheimer Wolff & Donnelly ;

Primary / Asst. Examiners: Leary, Louise N.;

INPADOC Legal Status: Show legal status actions

Family: None

First Claim:
Show all 9 claims
What is claimed is:     1. A method for determining the concentration of a heavy metal in a fluid containing both erythrocyte pyrimidine 5'-nucleotidase (Pyr-5'-N) and its isozyme deoxyribonucleotidase (d-5'-N) comprising measuring the ratio of the d-5'-N activity to the Pyr-5'-N activity and selecting the heavy metal concentration corresponding to the measured ratio.

Background / Summary: Show background / summary

Drawing Descriptions: Show drawing descriptions

Description: Show description

       
U.S. References: Go to Result Set: No U.S. references   |  No patents reference this one   |   Backward references (0)

       
Foreign References: None

Other Abstract Info: CHEMABS 130(14)178671K CHEMABS 130(14)178671K DERABS C1999-166637 DERABS C1999-166637

Other References:
  • Cook et al; "Drug and Chemical Toxicology"; (1988 Jun.) vol. 11(2), pp. 195-213 (Abstract). (19 pages)
  • Public Health Service. Preventing lead poisoning in young children: A statement by the Centers for Disease Control. Atlanta: CDC, 1984.
  • Bellinger D, Leviton A, Waternaux C, Needleman H, Rabinowitz M. Longitudinal analyses of prenatal and postnatal lead exposure and early cognitive development. N Engl J Med 1987; 316:1037-43. (7 pages) Cited by 3 patents
  • Agency for Toxic Substances and Disease Registry. The nature and extent of lead poisoning in children in the United States: A report to Congress. Atlanta:ATSDR, 1988.
  • Valentine WN, Fink K, Paglia DE, Harris SR, Adains WS. Hereditary hemolytic anemia with human erythrocyte pyrimidine 5'-nucleotidase deficiency. J Clin Invest 1974; 54:866-79. (14 pages)
  • Paglia DE, Valentine WN. Characteristics of a pyrimidine-specific 5'-nucleotidase in human erythrocytes. J Biol Chem 1975; 250: 7973-79. (7 pages) Cited by 2 patents
  • Paglia DE, Valentine WN, Keitt AS, Brockway RA, Nakatani M. Pyrimidine nucleotidase deficiency with active dephosphorylation of dTMP: Evidence for existence of thymidine nucleotidase in human erythrocytes. Blood 1983;62:1147-49. (3 pages)
  • Paglia DE, Valentine WN, Brockway RA. Identification of thymidine nucleotidase and deoxyribonucleotidase activities among normal isozymes of 5'-nucleotidase in human erythrocytes. Proc Natl Acad Sci (USA) 1984; 81:588-92. (5 pages)
  • Paglia DE, Valentine WN, Brockway RA, Nakatani M. Substrate specificity and pH sensitivity of deoxyribonucleotidase and pyrimidine nucleotidase activities in human hemolysates. Exp Hematol 1987; 15:1041-47. (7 pages)
  • Fiske CH, SubbaRow Y. The colorimetric determination of phosphorus. J Biol Chem 1925; 66:375-400.
  • Paglia DE, Valentine WN. Hereditary and acquired defects in the pyrimidine nucleotidase of human erythrocytes. Current Topics Hematol 1980; 3:75-109.
  • Paglia DE, Valentine WN, Dahlgren JG. Effects of low-level lead exposure on pyrimidine 5'-nucleotidase and other erythrocyte enzymes. Possible role of pyrimidine 5'-nucleotidase in the pathogenesis of lead-induced anemia. J Clin Invest 1975; 56:1164-69. (6 pages)
  • Paglia DE, Valentine WN, Fink K. Lead poisoning. Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides. J Clin Invest 1977; 60:1362-66. (5 pages)
  • Valentine WN, Paglia DE, Fink K, Madokoro G. Lead poisoning: Association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5'-nucleotidase deficiency and intraerythrocytic accumlulation of pyrimidines. J Clin Invest 1976; 58:926-32. (7 pages)
  • Buc HA, Kaplan JC. Red-cell pyrimidine 5'-nucleotidase and lead poisoning. Clin Chim Acta 1978; 87:49-55. (7 pages)
  • Angle CR, McIntire MS. Low level lead and inhibition of erythrocyte pyrimidine nucleotidase. Environ Res 1978; 17:296-302. (7 pages)
  • Brunet M, Vives-Corrons JL, Torra M, Rodamilans M, Pujades A, Corbella J, Pascual A. Assessment of erythrocyte pyrimidine 5'-nucleotidase activity in the detection and early diagnosis of lead poisoning. A comparison with zinc-protoporphyrin. Medicina Clin 1988; 91:521-24. (4 pages)
  • Mohammed-Brahim B, Buchet JP, Lauwerys R. Erythrocyte pyrimidine 5'-nucleotidase activity in workers exposed to lead, mercury or cadmium. Int Arch Occup Environ Health 1985; 55:247-255. (6 pages)
  • Ichiba M, Tomokuni K. Studies on erythrocyte pyrimidine 5'-nucleotidase (P5N) test and its evaluation in workers occupationally exposed to lead. Int Arch Occup Environ Health 1990; 62:305-10. (6 pages)
  • Cook L, Kubitschek C, Stohs S, Angle C. Erythrocyte pyrimidine 5'-nucleotidase and deoxynucleotidase isozymes: metallosensitivity and kinetics. Drug Chem Toxicol 1988; 11:195-213. (19 pages)
  • Ichiba M, Tomokuni K, Sugimoto K. Erythrocyte pyrimidine 5'-nucleotidase test for occupational lead exposure. Industr Health 1987; 25:195-213. (10 pages)
  • Fell GS. Lead toxicity: problems of definition and laboratory evaluation. Ann Clin Biochem 1984; 21:453-60. (8 pages)
  • Mitchell DG, Aldous KM, Ryan FJ. Mass screening for lead poisoning: capillary blood sampling and automated Delves-cup atomic-absorption analysis. N Y State J Med 1974; 74:1599-1603. (5 pages)
  • Piomelli S, Graziano J. Laboratory diagnosis of lead poisoning. Ped Clinics NA 1980; 27:843-53.
  • Wang ST, Pizzolato S, Peter F. Microsampling technique and determination of blood lead by Zeeman atomic absorption spectrophotometry. Sci Total Environ 1988; 71:37-43. (7 pages)
  • Jacobson B, Lockitch G, Quigley G. Improved sample preparation for accurate determination of low concentrations of lead in whole blood by graphite furnace analysis. Clin Chem 1991,.37:515-19. (5 pages) Cited by 2 patents [ISI abstract]
  • McElvaine MD, Orbach HG, Binder S, Blanksma LA, Maes EF, Krieg RM. Evaluation of the erythrocyte protoporphyrin test as a screen for elevated blood lead levels. J Pediatr 1991; 119:548-50. (3 pages) Cited by 2 patents [ISI abstract]
  • Hemberg S, Nikkanen J, Mellen G, Lilius H. δ-aminolevulinic acid dehydrase as a measure of lead exposure. Arch Envrion Health 1970; 21:140-45.


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