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 Title: |
US5932417:
Method of screening compounds for controlling capacitative calcium ion entry into mammalian cells
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Birnbaumer, Lutz; Los Angeles, CA
Zhu, Xi; Los Angeles, CA
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
1999-08-03
/ 1996-10-15
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| Application Number: |
US1996000729955
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| IPC Code: |
Advanced:
C07K 14/47;
C07K 14/705;
A61K 38/00;
Core:
C07K 14/435;
more...
IPC-7:
C07H 21/04;
C12N 15/00;
C12N 15/85;
C12Q 1/68;
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| ECLA Code: |
C07K14/47; C07K14/705;
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| U.S. Class: |
Current:
435/006;
435/069.1;
435/325;
435/375;
536/023.1;
536/024.5;
Original:
435/006;
435/069.1;
435/172.3;
435/325;
435/375;
536/023.1;
536/024.5;
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| Field of Search: |
435/004,6,69.1,325,172.3,375
536/23.1,24.5
514/044
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| Government Interest: |
This invention was made with government support under Grant No. HL-45198 from the National Institutes of Health. The Government has certain rights in this invention.
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| Priority Number: |
| 1996-10-15 |
US1996000729955 |
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| Abstract: |
A method for controlling capacitative calcium ion entry into a mammalian cell. The method is based on the discovery that mammalian transient receptor potential (trp) protein are essential for calcium ion entry. Two human trp proteins are disclosed. Htrp1 and Htrp3. The method involves treating cells with a trp-control agent to either raise or lower the amount of biologically active trp protein associated with the cell to thereby control capacitative calcium ion entry into the cell. Screening methods are also disclosed based upon using mammalian trp protein as a screening target.
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| Attorney, Agent or Firm: |
Oppenheimer Wolff & Donnelly LLP ;
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| Primary / Asst. Examiners: |
LeGuyader, John L.; Wang, Andrew
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| INPADOC Legal Status: |
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Family Legal Status Report
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| Designated Country: |
AU CA EP JP
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| Family: |
Show 3 known family members
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First Claim:
Show all 3 claims |
We claim:
1. A method of screening a compound for controlling capacitative calcium ion entry into mammalian cells, said method comprising the steps of:
- providing a cell culture which expresses a transient receptor potential protein which is selected from the group consisting of Htrm1 and Htrp3, said cell expressing said transient receptor potential protein to provide a naturally occurring level of biologically active transient receptor potential protein associated with said cell;
- exposing said cell culture to said compound; and
- determining if the exposure of said cell culture to said compound increases or decreases the expression of said transient receptor potential protein to thereby provide an indication of the compound's potential use in controlling capacitative calcium ion entry into mammalian cells.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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| Forward References: |
Show 1 U.S. patent(s) that reference this one
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| Foreign References: |
None
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| Other Abstract Info: |
CHEMABS 128(19)226220V
DERABS C1998-230269
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| Other References: |
Vaca et al., Activation of recombinant trp by thapsigargin in sf9 insect cells, American Journal of Physiology, vol. 267 (5, pt.1), pp. c1501-1505, 1994.
(5 pages)
[ISI abstract]
Verma et al., Gene therapy-promises, problems and prospects, Nature, vol. 389, pp. 239-242, Sep. 18, 1997.
(4 pages)
Cited by 1060 patents
[ISI abstract]
Zhu et al., Molecular cloning of a widely expressed human homologue for the Drosophila trp gene, FEBS Letters, vol. 373, pp. 193-198, Oct. 1995.
(6 pages)
Cited by 4 patents
[ISI abstract]
Mulligan, The basic science of gene therapy, Science, vol. 260, pp. 926-932, May 14, 1993.
(7 pages)
Cited by 319 patents
[ISI abstract]
Wes et al., TRPC1, a human homolog of a Drosophila store-operated channel, Proc. Natl. Acad. Sci., vol. 92, pp. 9652-9656, Oct. 1995.
(5 pages)
Cited by 4 patents
[ISI abstract]
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