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Title: US6010876: Clavanins
[ Derwent Title ]


Country: US United States of America

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39 pages

 
Inventor: Lehrer, Robert I.; Santa Monica, CA
Harwig, deceased, Sylvia L.; late of Woodland Hills, CA
Zhao, Chengquan; Los Angeles, CA
Lee, In-Hee; Seoul, Republic of Korea

Assignee: The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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Published / Filed: 2000-01-04 / 1996-11-06

Application Number: US1996000746160

IPC Code: Advanced: C07K 14/435; C12N 1/21; C12N 15/12; A61K 38/00;
Core: more...
IPC-7: C07K 14/435; C12N 1/21; C12N 15/12; C12N 15/63;

ECLA Code: C07K14/435A;

U.S. Class: Current: 435/069.1; 435/252.3; 435/320.1; 435/325; 435/419; 530/323; 530/326; 530/332; 536/023.5;
Original: 435/069.1; 536/023.5; 435/320.1; 435/325; 435/419; 435/252.3; 530/323; 530/326; 530/332;

Field of Search: 536/23.5 435/320.1,325,419,252.3 530/323,326,332

Government Interest:

ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
    This invention was made at least in part with funding from NIH grant numbers 1-PO1-AI-37945-01 and 5R37-AI-22839-10. The U.S. Government has certain rights in this invention.

Priority Number:
1996-11-06  US1996000746160

Abstract: Novel microbial peptides called clavanins are of the formula
    X'1 X2 B'3 X4 X5 U6 B7 X8 X9 B10 B11 X12 U13 Z14 X15 X16 B*17 U18 X19 U20 B21 X22 X23 ( 1)(SEQ ID NO: 1)
including the salts, esters, amides and acylated forms thereof
  • wherein X is a hydrophobic amino acid residue or modified form thereof;
  • X' is a small or a hydrophobic amino acid residue or a modified form thereof;
  • B is a basic amino acid residue or modified form thereof;
  • B' is basic or a polar/large amino acid residue or modified form thereof; and
  • B* is a basic or a hydrophobic amino acid residue or a modified form thereof;
  • U is a small amino acid residue or modified form thereof;
  • Z is a polar/large amino acid residue or modified form thereof.


Attorney, Agent or Firm: Morrison & Foerster LLP ;

Primary / Asst. Examiners: Pouty, Rebecca E.;

Maintenance Status: E1 Expired  Check current status

INPADOC Legal Status: Show legal status actions          Buy Now: Family Legal Status Report

Designated Country: CA EP JP 

Family: Show 4 known family members

First Claim:
Show all 4 claims
It is claimed:     1. A recombinant expression system for production of an antimicrobial peptide having an α-helical conformation and the amino acid sequence
    X'1 X2 B'3 X4 X5 U6 B7 X8 X9 B10 B11 X12 U13 Z14 X15 X16 B*17 U18 X19 U20 B21 X22 X23 ( 1) (SEQ ID NO:1)
including the salts, esters, amides and acylated forms thereof, wherein
  • X'1 is Val, Leu, Ile, or Ala;
  • X2 is Phe, Trp or Tyr;
  • B'3 is Asn, Gln, His, Lys or Arg;
  • X4 and X5 is each independently selected from the group consisting of Phe, Leu, Tyr, and Val;
  • U6 is Gly, Ser or Ala;
  • B7 is Lys or Arg;
  • X8 and X9 is each independently selected from the group consisting of Ile, Leu and Val;
  • B10 and B11 is each independently His, Lys or Arg;
  • X12 is Val, Ile, or Leu;
  • U13 is Ala, Ser or Gly;
  • Z14 is Asn or Gln;
  • X15 and X16 is each independently selected from the group consisting of Phe, Tyr, Trp, Val, Leu and Ile;
  • B*17 is His, Lys, Arg, Trp, Phe or Tyr or a modified form thereof;
  • U18 is Ala, Ser or Gly;
  • X19 is Phe, Tyr or Trp;
  • U20 is Gly, Ala or Ser;
  • B21 is His, Lys or Arg; and
  • each of X22 and X23 is Ile, Val, Leu, Phe, Tyr or Trp,
which expression system comprises a nucleotide sequence encoding said peptide operably linked to control sequences for effecting expression.


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U.S. References: Go to Result Set: No U.S. references   |  No patents reference this one   |   Backward references (0)

       
Foreign References:
Buy
PDF
Publication Date IPC Code Assignee   Title
Buy PDF- 6pp EP0403458 1990-12  A61K 38/00 KabiGen AB Novel polypeptides and their use 
Buy PDF- 84pp EP0472987 1992-03  A01N 63/02 ENICHEM PARTECIPAZIONI S.p.A. Antimicrobial peptides active against plant pathogens, their use and screening methods pertaining thereto 
Buy PDF- 123pp WO9311783 1993-06  A61K 37/02 MAGAININ PHARMACEUTICALS INC. COMPOSITION AND TREATMENT WITH BIOLOGICALLY ACTIVE PEPTIDES AND CHELATING AGENTS 


Other Abstract Info: CHEMABS 128(26)319289Q CHEMABS 132(18)233330S DERABS C1998-286858

Other References:
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  • Bensch, K. W. et al., "hBD-1; a novel β-defensin from human plasma," Febs Lett 368:331 (1995). (5 pages) Cited by 7 patents [ISI abstract]
  • Christensen, B. et al., "Channel-forming properties of cecropins and related model compounds incorporated into planar lipid membranes," Proc Natl Acad Sci USA 85:5072 (1988). (5 pages) Cited by 20 patents
  • Diamond, G. et al., "Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: Peptide isolation and cloning of a cDNA," Proc Natl Acad Sci USA 88:3952 (1991). (5 pages) Cited by 26 patents [ISI abstract]
  • Duclohier, H. et al., "Antimicrobial peptide magainin I from Xenopus skin forms anion-permeable channels in planar lipid bilayers," Biophys J 56:1017 (1989). (5 pages) Cited by 5 patents
  • Harwig, S. S. L. et al., "Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes," Febs Lett 342:281 (1994). (5 pages) Cited by 11 patents [ISI abstract]
  • Jones, D.E. et al., "Paneth Cells of the Human Small Intestine Express and Antimicrobial Peptide Gene," J Biol Chem 267:23216 (1992). (10 pages) Cited by 9 patents [ISI abstract]
  • Kokryakov, V. N. et al., "Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins," Febs Lett 231 (1993). (6 pages) Cited by 19 patents [ISI abstract]
  • Lehrer, R. I. et al., "DEFENSINS: Antimicrobial and Cytotoxic Peptides of Mammalian Cells," Ann Rev Immunol 11:105 (1992).
  • Nakamura, T. et al., "Tachyplesin, a Class of Antimicrobial Peptide from the Hemocytes of the Horseshoe Crab (Tachypleus tridentatus)," J Biol Chem 263:16709-16713 (1988). (5 pages) Cited by 28 patents
  • Patterson-Delafield, J. et al., "Microbial Cationic Proteins in Rabbit Alveolar Macrophages: a Potential Host Defense Mechanism," Infect Immun 30:180 (1980). (13 pages) Cited by 4 patents
  • Schonwetter, B. S. et al., "Epithelial Antibiotics Induced at Sites of Inflammation," Science 267:1645 (1995). (4 pages) Cited by 9 patents [ISI abstract]
  • Selsted, M. E. et al., "Purification, Primary Structures, and Antibacterial Activities of β-Defensins, a New Family of Antimicrobia Peptides from Bovine Neutrophils," J. Biol. Chem 268:6641.
  • Terry, A. S. et al., "The cDNA Sequence Coding for Prepro-PGS (Prepro-magainins) and Aspects of the Processing of This Prepro-polypeptide," J Biol Chem 263:5745 (1988). (7 pages) Cited by 5 patents
  • Zasloff, M. et al., "Antimicrobial activity of synthetic magainin peptides and several analogues," Proc Natl Acad Sci USA 85:910 (1988). (4 pages) Cited by 17 patents
  • Zasloff, M., "Magainins, a class of antimicrobial peptides from Xenopus skin: Isolation, characterization of two active forms, and partial cDNA sequence of a precursor," Proc Natl Acad Sci USA 84:5449 (1987). (5 pages) Cited by 38 patents


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