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 Title: |
US6153440:
Simultaneous measurement of free triiodothyronine and free thyroxine by equilibrium dialysis and immunoassay
[ Derwent Title ]
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| Country: |
US United States of America
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| Inventor: |
Chopra, Inder J.; Calabasas, CA
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| Assignee: |
The Regents of the University of California, Oakland, CA
other patents from UNIVERSITY OF CALIFORNIA, THE REGENTS OF (599425) (approx. 4,840)
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| Published / Filed: |
2000-11-28
/ 1998-09-23
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| Application Number: |
US1998000158872
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| IPC Code: |
Advanced:
G01N 33/78;
Core:
G01N 33/74;
IPC-7:
G01N 33/53;
G01N 33/552;
G01N 33/553;
G01N 33/554;
G01N 33/567;
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| ECLA Code: |
G01N33/78;
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| U.S. Class: |
Current:
436/500;
424/001.49;
435/005;
435/007.1;
435/007.22;
435/007.23;
435/007.32;
435/007.5;
436/504;
436/518;
436/526;
436/527;
436/538;
436/542;
Original:
436/500;
436/504;
436/526;
436/527;
436/518;
436/538;
436/542;
424/001;
424/111;
435/005;
435/007.1;
435/007.22;
435/007.32;
435/007.5;
435/007.23;
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| Field of Search: |
436/527,500,504,538,542,526,518
424/001,111
435/005,7.1,7.22,7.32,7.5,7.23
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| Priority Number: |
| 1998-09-23 |
US1998000158872 |
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| Abstract: |
The present invention provides methods for the simultaneous measurement of triiodothyronine (T3) and thyroxine (T4) in biological fluids such as serum by direct equilibrium dialysis and immunoassay. Specifically, the method comprises dialyzing the serum sample to equilibrium in a physiological buffer system so that the free T3 and the free T4 are separated from T3 and T4 bound to serum proteins. The method further comprises combining a measured quantity of the dialyzed serum sample having free T3 and free T4 with reagents comprising a measured quantity of T3 labelled with a detectable marker and a measured quantity of T4 labelled with a detectable marker; an anti-T3 antibody of sufficient specificity and in sufficient quantity to bind a measurable quantity of the free T3, and an anti-T4 antibody of sufficient specificity and in sufficient quantity to bind a measurable quantity of the free T4. The method then comprises allowing reaction of the free T3 and the free T4 and the labelled T3 and the labelled T4, with the anti-T3 antibody and the anti-T4 antibody to proceed substantially to equilibrium to thereby produce antibody bound labelled T3 and antibody bound labelled T4. Finally, the method comprises separating the unbound labelled T3 from the antibody bound labelled T3 and the unbound labelled T4 from the antibody bound labelled T4 ; and determining the levels of T3 and T4 in the sample by comparing relative amounts of antibody bound labelled T3 and T4 and unbound labelled T3 and T4. In one embodiment of this method, the assay is a radioimmunoassay.
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| Attorney, Agent or Firm: |
Gates & Cooper ;
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| Primary / Asst. Examiners: |
Minnifield, Nita; Baskar, Padma
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| INPADOC Legal Status: |
Show legal status actions
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| Family: |
None
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First Claim:
Show all 18 claims |
What is claimed is:
1. An immunoassay method for the simultaneous measurement of free thyroxine (T4) and free triiodo-L-thyronine (T3) thyroid hormone concentrations in a serum sample comprising the steps of:
- (a) dialyzing the serum sample to equilibrium in a physiological buffer system so that the free T3 and the free T4 are separated from T3 and T4 bound to serum proteins;
- (b) combining a measured quantity of the dialyzed serum sample having free T3 and free T4 with reagents comprising:
- (i) a measured quantity of T3 labelled with a detectable marker and a measured quantity of T4 labelled with a detectable marker;
- (ii) an anti-T3 antibody of sufficient specificity and in sufficient quantity to bind a measurable quantity of the free T3, and an anti-T4 antibody of sufficient specificity and in sufficient quantity to bind a measurable quantity of the free T4 ;
- (c) allowing reaction of the free T3 and the free T4 and the labelled T3 and the labelled T4, with the anti-T3 antibody and the anti-T4 antibody to proceed substantially to equilibrium to thereby produce antibody bound labelled T3 and antibody bound labelled T4 ;
- (d) separating the unbound labelled T3 from the antibody bound labelled T3 and the unbound labelled T4 from the antibody bound labelled T4 ; and
- (e) determining the concentrations of T3 and T4 in the sample by comparing relative amounts of antibody bound labelled T3 and T4 and unbound labelled T3 and T4.
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| Background / Summary: |
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| Drawing Descriptions: |
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| Description: |
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