Methods of screening modulators of opioid receptor activity (US6432652)


	Parent Case:			CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation-in-part of U.S. Ser. No. 08/403,260 filed Mar. 13, 1995, and now abandoned which is a continuation-in-part of U.S. Ser. No. 08/387,707 filed Feb. 13, 1995 and now U.S. Pat. No. 6,265,563 which is a national phase of PCT application US 93/07665 filed Aug. 13, 1993, which is a continuation-in-part of U.S. Ser. No. 07/929,200 filed Aug. 13, 1992 and now abandoned. The contents of these applications is incorporated herein by reference.
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	First Claim: Show all 3 claims			What is claimed is: 1. A method to screen a candidate substance for opioid agonist activity, which method comprises: (a) incubating cells displaying opioid receptor in the presence and absence of the candidate substance under conditions suitable for detection of activation of said opioid receptor, and (b) detecting the amount of activation of said opioid receptor in the presence as compared to the absence of said candidate substance; whereby an increase in the amount of activity in the presence, as compared to the absence of said candidate substance indicates that the substance is an agonist for said opioid receptor wherein said opioid receptor is produced and displayed on said cells by a method which comprises culturing said host cells which have been modified to contain an expression cassette which produces said opioid receptor in the host cells, said expression cassette comprising a nucleotide sequence encoding said opioid receptor operably linked to control sequences operable to provide expression in said host cells, and wherein said opioid receptor is a mammalian opioid receptor which is able to transduce a signal when contacted with an opioid agonist and is encoded by a nucleotide sequence which hybridizes under conditions of high stringency to the nucleotide sequence of SEQ. ID. NO: 7 or the complement thereof, wherein said conditions of high stringency comprise final wash steps of washing twice in 40 mM NaPO₄, pH 7.2; 0.5% BSA; 5% SDS; and 1 mM EDTA for one hour at 68° C. followed by final wash steps of washing twice in 40 mM NaPO₄, pH 7.2; 1% SDS; and 1 mM EDTA for one hour at 68° C.
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CHEMABS 137(12)164124S

Other References:

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Continuity Data:

Application Number

Filed

Notes

US1995000405271

1995-03-14

is a related to the prior publication

US20030124672A1 issued 2003-07-03 Opioid receptor genes

11855971

is a continuation of

US2002000290748

2002-11-07